TY - JOUR
T1 - Ethanol Vapor Imaging System "sniffer Camera" for Evaluation of Alcohol Metabolism from Breath and Palm Skin Gas
AU - Arakawa, Takahiro
AU - Iitani, Kenta
AU - Sato, Toshiyuki
AU - Toma, Koji
AU - Mitsubayashi, Kohji
N1 - Publisher Copyright:
© 2016 Published by Elsevier Ltd.
PY - 2016
Y1 - 2016
N2 - Breath and body odors are composed of various volatile organic compounds in human transpiration. The gaseous components are related to disease and human metabolic function. Therefore, a novel two-dimensional fluorometric imaging system, known as a "sniffer camera" for gaseous ethanol emissions from human breath and palm skin was constructed and tested. The imaging system measures ethanol vapor concentrations as intensities of fluorescence through an enzymatic reaction induced by alcohol dehydrogenase (ADH). The imaging system consisted of a enzyme immobilized cotton mesh, a multi UV-LED excitation sheet and a high-sensitive CCD camera. This imaging system utilized ADH for recognition of ethanol vapor. It measured ethanol vapor by analysing fluorescent intensity of nicotinamide adenine dinucleotide (NADH), which is produced by an enzymatic reaction on a cotton mesh. This NADH fluorometric imaging system achieved the two-dimensional real-time imaging of ethanol vapor distribution from 0.5 to 200 ppm. The system showed a rapidly and accurately responses and a visible measurement, which could lead an analysis to metabolism function.
AB - Breath and body odors are composed of various volatile organic compounds in human transpiration. The gaseous components are related to disease and human metabolic function. Therefore, a novel two-dimensional fluorometric imaging system, known as a "sniffer camera" for gaseous ethanol emissions from human breath and palm skin was constructed and tested. The imaging system measures ethanol vapor concentrations as intensities of fluorescence through an enzymatic reaction induced by alcohol dehydrogenase (ADH). The imaging system consisted of a enzyme immobilized cotton mesh, a multi UV-LED excitation sheet and a high-sensitive CCD camera. This imaging system utilized ADH for recognition of ethanol vapor. It measured ethanol vapor by analysing fluorescent intensity of nicotinamide adenine dinucleotide (NADH), which is produced by an enzymatic reaction on a cotton mesh. This NADH fluorometric imaging system achieved the two-dimensional real-time imaging of ethanol vapor distribution from 0.5 to 200 ppm. The system showed a rapidly and accurately responses and a visible measurement, which could lead an analysis to metabolism function.
KW - alcohol dehydrogenase
KW - ethanol
KW - ethanol vapor
KW - fluorescence
KW - NADH
KW - skin gas
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U2 - 10.1016/j.proeng.2016.11.514
DO - 10.1016/j.proeng.2016.11.514
M3 - Conference article
AN - SCOPUS:85009957911
VL - 168
SP - 522
EP - 528
JO - Procedia Engineering
JF - Procedia Engineering
SN - 1877-7058
T2 - 30th Eurosensors Conference, Eurosensors 2016
Y2 - 4 September 2016 through 7 September 2016
ER -