Evaluation of Polyphenolic Content and Potential Antioxidant Activity of Japanese Cultivars of Peaches, Prunes, and Plums Based on Reversed- and Normal-Phase HPLC and Principal Component Analyses

Takayuki Goto, Mina Obara, Shiori Aoki, Katsuhiko Okazawa, Kunihiko Konisho, Naomi Osakabe, Toshihiko Shoji

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the amount of polyphenols present in three types of stone fruits (peaches, plums, and prunes) that were produced in Japan during 2018 and 2019 using reversed-phase high-performance liquid chromatography (HPLC) with photodiode arrays and by normal-phase HPLC with fluorescence-based detection. Principal component analysis revealed the characteristics of the three fruit types: the major contributors of polyphenolic content in peaches were catechin, procyanidin B1, and 5-caffeoylquinic acid; those in plums, procyanidin B4, epicatechin, and procyanidin B5; and those in prunes, 3-caffeoylquinic acid and peonidine-3-rutinoside. Next, their antioxidative activity was assessed using hydrophilic oxygen radical absorbance capacity (H-ORAC). The sum of all polyphenol contents was significantly correlated with H-ORAC values (R2 = 0.6205). Both the concentrations of flavan-3-ols and procyanidins measured by reversed- and normal-phase HPLC were significantly correlated with H-ORAC values (R2 = 0.7248 and 0.7036, respectively). This work provides a reference for further investigations of stone fruit functions.

Original languageEnglish
Pages (from-to)2019-2029
Number of pages11
JournalACS Food Science and Technology
Volume1
Issue number10
DOIs
Publication statusPublished - 2021 Nov 19

Keywords

  • anthocyanins
  • flavan-3-ols
  • H-ORAC
  • hydroxycinnamates
  • normal-phase HPLC
  • polyphenols
  • principal component analysis
  • procyanidins
  • reversed-phase HPLC

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry
  • Organic Chemistry
  • Chemistry (miscellaneous)

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