TY - JOUR
T1 - Evaluation of Polyphenolic Content and Potential Antioxidant Activity of Japanese Cultivars of Peaches, Prunes, and Plums Based on Reversed- and Normal-Phase HPLC and Principal Component Analyses
AU - Goto, Takayuki
AU - Obara, Mina
AU - Aoki, Shiori
AU - Okazawa, Katsuhiko
AU - Konisho, Kunihiko
AU - Osakabe, Naomi
AU - Shoji, Toshihiko
N1 - Funding Information:
This work was supported by the Cross-ministerial Strategic Innovation Promotion Program (SIP), “Technologies for Smart Bio-industry and Agriculture” (funding agency: Bio-oriented Technology Research Advancement Institution, NARO, Japan).
Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/11/19
Y1 - 2021/11/19
N2 - We investigated the amount of polyphenols present in three types of stone fruits (peaches, plums, and prunes) that were produced in Japan during 2018 and 2019 using reversed-phase high-performance liquid chromatography (HPLC) with photodiode arrays and by normal-phase HPLC with fluorescence-based detection. Principal component analysis revealed the characteristics of the three fruit types: the major contributors of polyphenolic content in peaches were catechin, procyanidin B1, and 5-caffeoylquinic acid; those in plums, procyanidin B4, epicatechin, and procyanidin B5; and those in prunes, 3-caffeoylquinic acid and peonidine-3-rutinoside. Next, their antioxidative activity was assessed using hydrophilic oxygen radical absorbance capacity (H-ORAC). The sum of all polyphenol contents was significantly correlated with H-ORAC values (R2 = 0.6205). Both the concentrations of flavan-3-ols and procyanidins measured by reversed- and normal-phase HPLC were significantly correlated with H-ORAC values (R2 = 0.7248 and 0.7036, respectively). This work provides a reference for further investigations of stone fruit functions.
AB - We investigated the amount of polyphenols present in three types of stone fruits (peaches, plums, and prunes) that were produced in Japan during 2018 and 2019 using reversed-phase high-performance liquid chromatography (HPLC) with photodiode arrays and by normal-phase HPLC with fluorescence-based detection. Principal component analysis revealed the characteristics of the three fruit types: the major contributors of polyphenolic content in peaches were catechin, procyanidin B1, and 5-caffeoylquinic acid; those in plums, procyanidin B4, epicatechin, and procyanidin B5; and those in prunes, 3-caffeoylquinic acid and peonidine-3-rutinoside. Next, their antioxidative activity was assessed using hydrophilic oxygen radical absorbance capacity (H-ORAC). The sum of all polyphenol contents was significantly correlated with H-ORAC values (R2 = 0.6205). Both the concentrations of flavan-3-ols and procyanidins measured by reversed- and normal-phase HPLC were significantly correlated with H-ORAC values (R2 = 0.7248 and 0.7036, respectively). This work provides a reference for further investigations of stone fruit functions.
KW - anthocyanins
KW - flavan-3-ols
KW - H-ORAC
KW - hydroxycinnamates
KW - normal-phase HPLC
KW - polyphenols
KW - principal component analysis
KW - procyanidins
KW - reversed-phase HPLC
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U2 - 10.1021/acsfoodscitech.1c00357
DO - 10.1021/acsfoodscitech.1c00357
M3 - Article
AN - SCOPUS:85127273252
VL - 1
SP - 2019
EP - 2029
JO - ACS Food Science and Technology
JF - ACS Food Science and Technology
SN - 2692-1944
IS - 10
ER -