Abstract
The transport of intensity equation (TIE) is a convenient method of obtaining a potential distribution, as it requires only three transmission electron microscopy images with different amounts of defocus. However, the spatial resolution of the TIE phase map has not yet been evaluated experimentally. In this study, we investigated the phase distribution of spherical gold nanoparticles and its dependence on the defocus difference and found that the spatial resolution was finer than 2 nm, even for a defocus difference of 4 µm. Theoretical calculations reproduced the experimental results well.
| Original language | English |
|---|---|
| Pages (from-to) | 395-400 |
| Number of pages | 6 |
| Journal | Microscopy |
| Volume | 64 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2015 Dec 1 |
| Externally published | Yes |
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Keywords
- Gold nanoparticle
- Mean inner potential
- Phase retrieval
- Spatial resolution
- Transport of intensity equation
ASJC Scopus subject areas
- Structural Biology
- Medicine(all)
- Instrumentation
- Radiology Nuclear Medicine and imaging
Cite this
Experimental evaluation of spatial resolution in phase maps retrieved by transport of intensity equation. / Zhang, Xiaobin; Oshima, Yoshifumi.
In: Microscopy, Vol. 64, No. 6, 01.12.2015, p. 395-400.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Experimental evaluation of spatial resolution in phase maps retrieved by transport of intensity equation
AU - Zhang, Xiaobin
AU - Oshima, Yoshifumi
PY - 2015/12/1
Y1 - 2015/12/1
N2 - The transport of intensity equation (TIE) is a convenient method of obtaining a potential distribution, as it requires only three transmission electron microscopy images with different amounts of defocus. However, the spatial resolution of the TIE phase map has not yet been evaluated experimentally. In this study, we investigated the phase distribution of spherical gold nanoparticles and its dependence on the defocus difference and found that the spatial resolution was finer than 2 nm, even for a defocus difference of 4 µm. Theoretical calculations reproduced the experimental results well.
AB - The transport of intensity equation (TIE) is a convenient method of obtaining a potential distribution, as it requires only three transmission electron microscopy images with different amounts of defocus. However, the spatial resolution of the TIE phase map has not yet been evaluated experimentally. In this study, we investigated the phase distribution of spherical gold nanoparticles and its dependence on the defocus difference and found that the spatial resolution was finer than 2 nm, even for a defocus difference of 4 µm. Theoretical calculations reproduced the experimental results well.
KW - Gold nanoparticle
KW - Mean inner potential
KW - Phase retrieval
KW - Spatial resolution
KW - Transport of intensity equation
UR - http://www.scopus.com/inward/record.url?scp=84952770092&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84952770092&partnerID=8YFLogxK
U2 - 10.1093/jmicro/dfv045
DO - 10.1093/jmicro/dfv045
M3 - Article
AN - SCOPUS:84952770092
VL - 64
SP - 395
EP - 400
JO - Microscopy (Oxford, England)
JF - Microscopy (Oxford, England)
SN - 2050-5698
IS - 6
ER -