Experimental evaluation of spatial resolution in phase maps retrieved by transport of intensity equation

Xiaobin Zhang; Yoshifumi Oshima

doi:10.1093/jmicro/dfv045

Experimental evaluation of spatial resolution in phase maps retrieved by transport of intensity equation

Xiaobin Zhang, Yoshifumi Oshima

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The transport of intensity equation (TIE) is a convenient method of obtaining a potential distribution, as it requires only three transmission electron microscopy images with different amounts of defocus. However, the spatial resolution of the TIE phase map has not yet been evaluated experimentally. In this study, we investigated the phase distribution of spherical gold nanoparticles and its dependence on the defocus difference and found that the spatial resolution was finer than 2 nm, even for a defocus difference of 4 µm. Theoretical calculations reproduced the experimental results well.

Original language	English
Pages (from-to)	395-400
Number of pages	6
Journal	Microscopy
Volume	64
Issue number	6
DOIs	https://doi.org/10.1093/jmicro/dfv045
Publication status	Published - 2015 Dec 1
Externally published	Yes

Keywords

Gold nanoparticle
Mean inner potential
Phase retrieval
Spatial resolution
Transport of intensity equation

ASJC Scopus subject areas

Structural Biology
Medicine(all)
Instrumentation
Radiology Nuclear Medicine and imaging

Access to Document

10.1093/jmicro/dfv045

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AB - The transport of intensity equation (TIE) is a convenient method of obtaining a potential distribution, as it requires only three transmission electron microscopy images with different amounts of defocus. However, the spatial resolution of the TIE phase map has not yet been evaluated experimentally. In this study, we investigated the phase distribution of spherical gold nanoparticles and its dependence on the defocus difference and found that the spatial resolution was finer than 2 nm, even for a defocus difference of 4 µm. Theoretical calculations reproduced the experimental results well.

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