Experimental examination of the characteristics of bright-field scanning confocal electron microscopy images

Ayako Hashimoto, Kazutaka Mitsuishi, Masayuki Shimojo, Yufang Zhu, Masaki Takeguchi

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3 Citations (Scopus)


We experimentally examined the characteristics of bright-field (BF) scanning confocal electron microscopy (SCEM) images by changing the observation conditions and comparing the images with those obtained by BF transmission electron microscopy (TEM) and BF scanning TEM (STEM) modes. The observation of 5-nm-diameter Au nanoparticles demonstrated that BF-SCEM produces object elongation of more than 2000 nm along the optical axis, as do BF-TEM and BF-STEM. We demonstrated the relationship between elongation length and geometric effects such as convergence and collection angles of a probe and the lateral size of an object; the relationship is consistent with previous theoretical prediction. Further, we observed interesting features that are seen only in the BF-SCEM images; the film contrast was strongly enhanced, compared with that of BF-STEM. In addition, a bright contrast appeared around the object position in the elongated images. Using this characteristic, we could determine the object position and structure.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalJournal of Electron Microscopy
Issue number3
Publication statusPublished - 2011 Jun 1



  • bright-field imaging
  • confocal imaging
  • depth sectioning
  • scanning confocal electron microscopy
  • stage-scanning system
  • three-dimensional imaging

ASJC Scopus subject areas

  • Instrumentation

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