Expression and characterization of Xenopus laevis SRY-related cDNAs, xSox17α1, xSox17α2, xSox18α and xSox18β

Masanori Hasegawa, Yoshiki Hiraoka, Jun Hagiudal, Motoyuki Ogawa, Sadakazu Aiso

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Sox is a large family of genes related to the sex-determining region Y gene (designated as the SRY gene). Sox genes encoding DNA-binding transcriptional factors are found in many animals and are involved in developmental events. In this study, we newly isolated and sequenced novel Sox cDNAs from African clawed frog (Xenopus laevis). Five clones isolated here were classified into four distinct Sox genes designated as xSox17α1, xSox17α2, xSox18α and xSox18β. All four belong to a subtype of SOX family, type F. The cDNA xSox17α1 contains essentially the same nucleotide sequence as that identified as Sox17α in a previous work (Cell 91 (1997) 397), whereas xSox17α2 is a distinct gene with high homology to xSox17α1. The clones, xSox18α and xSox18β, are highly homologous to each other over the entire nucleotide sequences. The xSox18α and xSox18β genes encode 363 and 361 amino acids, respectively. Genomic Southern hybridization analysis showed the existence of two copies of the xSox18. Northern analysis indicated that the xSox18 gene was expressed in the spleen and kidney and the size of the transcript was estimated to be 2.4 knt. Electrophoretic mobility shift assays indicated that recombinant xSox18 polypeptide was capable of binding to the HMG consensus nucleotide sequence, AACAAT.

Original languageEnglish
Pages (from-to)163-172
Number of pages10
JournalGene
Volume290
Issue number1-2
DOIs
Publication statusPublished - 2002 May 15

Keywords

  • DNA binding
  • Embryogenesis
  • HMG box
  • Nucleotide sequence
  • SOX
  • SRY
  • Xenopus laevis

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Expression and characterization of Xenopus laevis SRY-related cDNAs, xSox17α1, xSox17α2, xSox18α and xSox18β'. Together they form a unique fingerprint.

  • Cite this