Expression and characterization of Xenopus laevis SRY-related cDNAs, xSox17α1, xSox17α2, xSox18α and xSox18β

Masanori Hasegawa, Yoshiki Hiraoka, Jun Hagiudal, Motoyuki Ogawa, Sadakazu Aiso

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Sox is a large family of genes related to the sex-determining region Y gene (designated as the SRY gene). Sox genes encoding DNA-binding transcriptional factors are found in many animals and are involved in developmental events. In this study, we newly isolated and sequenced novel Sox cDNAs from African clawed frog (Xenopus laevis). Five clones isolated here were classified into four distinct Sox genes designated as xSox17α1, xSox17α2, xSox18α and xSox18β. All four belong to a subtype of SOX family, type F. The cDNA xSox17α1 contains essentially the same nucleotide sequence as that identified as Sox17α in a previous work (Cell 91 (1997) 397), whereas xSox17α2 is a distinct gene with high homology to xSox17α1. The clones, xSox18α and xSox18β, are highly homologous to each other over the entire nucleotide sequences. The xSox18α and xSox18β genes encode 363 and 361 amino acids, respectively. Genomic Southern hybridization analysis showed the existence of two copies of the xSox18. Northern analysis indicated that the xSox18 gene was expressed in the spleen and kidney and the size of the transcript was estimated to be 2.4 knt. Electrophoretic mobility shift assays indicated that recombinant xSox18 polypeptide was capable of binding to the HMG consensus nucleotide sequence, AACAAT.

Original languageEnglish
Pages (from-to)163-172
Number of pages10
JournalGene
Volume290
Issue number1-2
DOIs
Publication statusPublished - 2002 May 15
Externally publishedYes

Keywords

  • DNA binding
  • Embryogenesis
  • HMG box
  • Nucleotide sequence
  • SOX
  • SRY
  • Xenopus laevis

ASJC Scopus subject areas

  • Genetics

Cite this

Expression and characterization of Xenopus laevis SRY-related cDNAs, xSox17α1, xSox17α2, xSox18α and xSox18β. / Hasegawa, Masanori; Hiraoka, Yoshiki; Hagiudal, Jun; Ogawa, Motoyuki; Aiso, Sadakazu.

In: Gene, Vol. 290, No. 1-2, 15.05.2002, p. 163-172.

Research output: Contribution to journalArticle

Hasegawa, Masanori ; Hiraoka, Yoshiki ; Hagiudal, Jun ; Ogawa, Motoyuki ; Aiso, Sadakazu. / Expression and characterization of Xenopus laevis SRY-related cDNAs, xSox17α1, xSox17α2, xSox18α and xSox18β. In: Gene. 2002 ; Vol. 290, No. 1-2. pp. 163-172.
@article{5335d14c063446c78814dabf3cbfb490,
title = "Expression and characterization of Xenopus laevis SRY-related cDNAs, xSox17α1, xSox17α2, xSox18α and xSox18β",
abstract = "Sox is a large family of genes related to the sex-determining region Y gene (designated as the SRY gene). Sox genes encoding DNA-binding transcriptional factors are found in many animals and are involved in developmental events. In this study, we newly isolated and sequenced novel Sox cDNAs from African clawed frog (Xenopus laevis). Five clones isolated here were classified into four distinct Sox genes designated as xSox17α1, xSox17α2, xSox18α and xSox18β. All four belong to a subtype of SOX family, type F. The cDNA xSox17α1 contains essentially the same nucleotide sequence as that identified as Sox17α in a previous work (Cell 91 (1997) 397), whereas xSox17α2 is a distinct gene with high homology to xSox17α1. The clones, xSox18α and xSox18β, are highly homologous to each other over the entire nucleotide sequences. The xSox18α and xSox18β genes encode 363 and 361 amino acids, respectively. Genomic Southern hybridization analysis showed the existence of two copies of the xSox18. Northern analysis indicated that the xSox18 gene was expressed in the spleen and kidney and the size of the transcript was estimated to be 2.4 knt. Electrophoretic mobility shift assays indicated that recombinant xSox18 polypeptide was capable of binding to the HMG consensus nucleotide sequence, AACAAT.",
keywords = "DNA binding, Embryogenesis, HMG box, Nucleotide sequence, SOX, SRY, Xenopus laevis",
author = "Masanori Hasegawa and Yoshiki Hiraoka and Jun Hagiudal and Motoyuki Ogawa and Sadakazu Aiso",
year = "2002",
month = "5",
day = "15",
doi = "10.1016/S0378-1119(02)00554-1",
language = "English",
volume = "290",
pages = "163--172",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Expression and characterization of Xenopus laevis SRY-related cDNAs, xSox17α1, xSox17α2, xSox18α and xSox18β

AU - Hasegawa, Masanori

AU - Hiraoka, Yoshiki

AU - Hagiudal, Jun

AU - Ogawa, Motoyuki

AU - Aiso, Sadakazu

PY - 2002/5/15

Y1 - 2002/5/15

N2 - Sox is a large family of genes related to the sex-determining region Y gene (designated as the SRY gene). Sox genes encoding DNA-binding transcriptional factors are found in many animals and are involved in developmental events. In this study, we newly isolated and sequenced novel Sox cDNAs from African clawed frog (Xenopus laevis). Five clones isolated here were classified into four distinct Sox genes designated as xSox17α1, xSox17α2, xSox18α and xSox18β. All four belong to a subtype of SOX family, type F. The cDNA xSox17α1 contains essentially the same nucleotide sequence as that identified as Sox17α in a previous work (Cell 91 (1997) 397), whereas xSox17α2 is a distinct gene with high homology to xSox17α1. The clones, xSox18α and xSox18β, are highly homologous to each other over the entire nucleotide sequences. The xSox18α and xSox18β genes encode 363 and 361 amino acids, respectively. Genomic Southern hybridization analysis showed the existence of two copies of the xSox18. Northern analysis indicated that the xSox18 gene was expressed in the spleen and kidney and the size of the transcript was estimated to be 2.4 knt. Electrophoretic mobility shift assays indicated that recombinant xSox18 polypeptide was capable of binding to the HMG consensus nucleotide sequence, AACAAT.

AB - Sox is a large family of genes related to the sex-determining region Y gene (designated as the SRY gene). Sox genes encoding DNA-binding transcriptional factors are found in many animals and are involved in developmental events. In this study, we newly isolated and sequenced novel Sox cDNAs from African clawed frog (Xenopus laevis). Five clones isolated here were classified into four distinct Sox genes designated as xSox17α1, xSox17α2, xSox18α and xSox18β. All four belong to a subtype of SOX family, type F. The cDNA xSox17α1 contains essentially the same nucleotide sequence as that identified as Sox17α in a previous work (Cell 91 (1997) 397), whereas xSox17α2 is a distinct gene with high homology to xSox17α1. The clones, xSox18α and xSox18β, are highly homologous to each other over the entire nucleotide sequences. The xSox18α and xSox18β genes encode 363 and 361 amino acids, respectively. Genomic Southern hybridization analysis showed the existence of two copies of the xSox18. Northern analysis indicated that the xSox18 gene was expressed in the spleen and kidney and the size of the transcript was estimated to be 2.4 knt. Electrophoretic mobility shift assays indicated that recombinant xSox18 polypeptide was capable of binding to the HMG consensus nucleotide sequence, AACAAT.

KW - DNA binding

KW - Embryogenesis

KW - HMG box

KW - Nucleotide sequence

KW - SOX

KW - SRY

KW - Xenopus laevis

UR - http://www.scopus.com/inward/record.url?scp=0037094378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037094378&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(02)00554-1

DO - 10.1016/S0378-1119(02)00554-1

M3 - Article

VL - 290

SP - 163

EP - 172

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -