Fast electron transfer between glucose oxidase and electrodes via phenothiazine mediators with poly(ethylene oxide) spacers attached to the enzyme surface

Kazumichi Ban, Takeshi Ueki, Yoshinori Tamada, Takahiro Saito, Shinichiro Imabayashi, Masayoshi Watanabe

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A series of glucose oxidase hybrids capable of a direct electrical communication with electrodes is synthesized by covalently bonding phenothiazine mediators to surface lysine residues via poly(ethylene oxide) spacers with different lengths. The hybrid with the optimum length of spacer chain exhibits the fast electron transfer between the redox center of enzyme and electrodes. This fast electron transfer in the hybrids realizes a large catalytic current comparable to that for the corresponding freely diffusing mediator systems.

Original languageEnglish
Pages (from-to)649-653
Number of pages5
JournalElectrochemistry Communications
Volume3
Issue number11
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Glucose Oxidase
Glucose oxidase
Polyethylene oxides
Enzymes
Electrodes
Electrons
Lysine
Communication
phenothiazine
Oxidation-Reduction

Keywords

  • Bioconjugates
  • Electron transfer
  • Glucose oxidase
  • Phenothiazine
  • Poly(ethylene oxide)

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Analytical Chemistry
  • Electrochemistry

Cite this

Fast electron transfer between glucose oxidase and electrodes via phenothiazine mediators with poly(ethylene oxide) spacers attached to the enzyme surface. / Ban, Kazumichi; Ueki, Takeshi; Tamada, Yoshinori; Saito, Takahiro; Imabayashi, Shinichiro; Watanabe, Masayoshi.

In: Electrochemistry Communications, Vol. 3, No. 11, 2001, p. 649-653.

Research output: Contribution to journalArticle

@article{5bb7eef0e61b4181bdaa3d63a1a2579b,
title = "Fast electron transfer between glucose oxidase and electrodes via phenothiazine mediators with poly(ethylene oxide) spacers attached to the enzyme surface",
abstract = "A series of glucose oxidase hybrids capable of a direct electrical communication with electrodes is synthesized by covalently bonding phenothiazine mediators to surface lysine residues via poly(ethylene oxide) spacers with different lengths. The hybrid with the optimum length of spacer chain exhibits the fast electron transfer between the redox center of enzyme and electrodes. This fast electron transfer in the hybrids realizes a large catalytic current comparable to that for the corresponding freely diffusing mediator systems.",
keywords = "Bioconjugates, Electron transfer, Glucose oxidase, Phenothiazine, Poly(ethylene oxide)",
author = "Kazumichi Ban and Takeshi Ueki and Yoshinori Tamada and Takahiro Saito and Shinichiro Imabayashi and Masayoshi Watanabe",
year = "2001",
doi = "10.1016/S1388-2481(01)00236-3",
language = "English",
volume = "3",
pages = "649--653",
journal = "Electrochemistry Communications",
issn = "1388-2481",
publisher = "Elsevier Inc.",
number = "11",

}

TY - JOUR

T1 - Fast electron transfer between glucose oxidase and electrodes via phenothiazine mediators with poly(ethylene oxide) spacers attached to the enzyme surface

AU - Ban, Kazumichi

AU - Ueki, Takeshi

AU - Tamada, Yoshinori

AU - Saito, Takahiro

AU - Imabayashi, Shinichiro

AU - Watanabe, Masayoshi

PY - 2001

Y1 - 2001

N2 - A series of glucose oxidase hybrids capable of a direct electrical communication with electrodes is synthesized by covalently bonding phenothiazine mediators to surface lysine residues via poly(ethylene oxide) spacers with different lengths. The hybrid with the optimum length of spacer chain exhibits the fast electron transfer between the redox center of enzyme and electrodes. This fast electron transfer in the hybrids realizes a large catalytic current comparable to that for the corresponding freely diffusing mediator systems.

AB - A series of glucose oxidase hybrids capable of a direct electrical communication with electrodes is synthesized by covalently bonding phenothiazine mediators to surface lysine residues via poly(ethylene oxide) spacers with different lengths. The hybrid with the optimum length of spacer chain exhibits the fast electron transfer between the redox center of enzyme and electrodes. This fast electron transfer in the hybrids realizes a large catalytic current comparable to that for the corresponding freely diffusing mediator systems.

KW - Bioconjugates

KW - Electron transfer

KW - Glucose oxidase

KW - Phenothiazine

KW - Poly(ethylene oxide)

UR - http://www.scopus.com/inward/record.url?scp=0034753671&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034753671&partnerID=8YFLogxK

U2 - 10.1016/S1388-2481(01)00236-3

DO - 10.1016/S1388-2481(01)00236-3

M3 - Article

VL - 3

SP - 649

EP - 653

JO - Electrochemistry Communications

JF - Electrochemistry Communications

SN - 1388-2481

IS - 11

ER -