TY - JOUR
T1 - Fiber optic biosniffer (biochemical gas sensor) for gaseous dimethyl sulfide
AU - Saito, Hirokazu
AU - Hashimoto, Yuki
AU - Minamide, Takeshi
AU - Kon, Takuo
AU - Toma, Koji
AU - Arakawa, Takahiro
AU - Mitsubayashi, Kohji
N1 - Funding Information:
This work was supported by the Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research System, Japan Science and Technology Agency (JST) and Ministry of Education, Culture, Sports, Science and Technology (MEXT) Special Funds for Education and Research "Advanced Research Program in Sensing Biology".
Publisher Copyright:
© MYU K.K.
PY - 2016
Y1 - 2016
N2 - An optical biosniffer for dimethyl sulfide (DMS; seaweed-odor substance) was constructed by immobilizing flavin-containing monooxygenase type 3 (FMO3) onto the tip of a fiber optic oxygen sensor with an oxygen-sensitive ruthenium organic complex (excitation: 470 nm, fluorescence: 600 nm) with a tube ring. A reaction unit for circulating buffer solution was applied to the tip of the sensor device. After the experiment in the liquid phase, the sniffer device was applied to gas analysis using a gas flow measurement system with a gas generator. The optical device could be applied to detect the oxygen consumption induced by the FMO3 enzymatic reaction with DMS application. The sensor in the liquid phase was used to measure DMS solution from 0.52 to 7.32 mmol/l, with a cyclic reaction with ascorbic acid (AsA) as the reducing reagent. Then, the sniffer device with the reaction unit was calibrated against DMS vapor from 2.1-126 ppm with good gas selectivity based on the FMO3 substrate specificity. The device could be used to monitor the concentration change of gaseous DMS using the reaction unit, in which the substrate and product substances flush out at the sensor tip and the enzyme membrane.
AB - An optical biosniffer for dimethyl sulfide (DMS; seaweed-odor substance) was constructed by immobilizing flavin-containing monooxygenase type 3 (FMO3) onto the tip of a fiber optic oxygen sensor with an oxygen-sensitive ruthenium organic complex (excitation: 470 nm, fluorescence: 600 nm) with a tube ring. A reaction unit for circulating buffer solution was applied to the tip of the sensor device. After the experiment in the liquid phase, the sniffer device was applied to gas analysis using a gas flow measurement system with a gas generator. The optical device could be applied to detect the oxygen consumption induced by the FMO3 enzymatic reaction with DMS application. The sensor in the liquid phase was used to measure DMS solution from 0.52 to 7.32 mmol/l, with a cyclic reaction with ascorbic acid (AsA) as the reducing reagent. Then, the sniffer device with the reaction unit was calibrated against DMS vapor from 2.1-126 ppm with good gas selectivity based on the FMO3 substrate specificity. The device could be used to monitor the concentration change of gaseous DMS using the reaction unit, in which the substrate and product substances flush out at the sensor tip and the enzyme membrane.
KW - DMS vapor
KW - Flavin-containing monooxygenase
KW - Optical biosniffer
KW - Oxygen-sensitive optical fiber
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U2 - 10.18494/SAM.2016.1291
DO - 10.18494/SAM.2016.1291
M3 - Article
AN - SCOPUS:85006434665
VL - 28
SP - 1295
EP - 1301
JO - Sensors and Materials
JF - Sensors and Materials
SN - 0914-4935
IS - 12
ER -