Abstract
Periods of skeletal muscle disuse, for example due to a sedentary lifestyle or bed rest, are associated with aging and can lead to muscle atrophy. We previously found that the flavan 3-ol fraction derived from cocoa (FL) enhanced energy expenditure with metabolic changes in skeletal muscle. In the present study, we examined the effect of FL on disuse muscle atrophy induced by hindlimb suspension in mice. Male C57BL/6J mice were assigned to four groups as follows: unsuspended-vehicle, unsuspended-FL, suspended-vehicle, and suspended-FL. Mice in the vehicle treatment groups were administered distilled water and those in the FL treatment groups were dosed with FL (50 mg/kg/day) for 2 weeks. The weights of the gastrocnemius (GC), tibialis anterior (TA), and soleus (SOL), but not the extensor digitorum longus (EDL), decreased significantly in mice with hindlimb suspension (− 11.8%, − 16.5%, and − 41.0%, respectively). This reduction in GC, TA, and SOL mass was inhibited by FL (− 5.3%, + 2.0%, and − 16.6%, respectively). The FL increased the EDL weight > 20% with or without hindlimb suspension. The protein level of the ubiquitin ligase, muscle ring finger-1, in the SOL was significantly increased by hindlimb suspension, but inhibited by treatment with FL. Protein expression of p70S6 kinase in the SOL was significantly decreased by hindlimb suspension, and FL treatment inhibited this change. These results suggested that FL delayed disuse muscle atrophy by metabolic alteration.
| Language | English |
|---|---|
| Pages | 120-123 |
| Number of pages | 4 |
| Journal | Experimental Gerontology |
| Volume | 98 |
| DOIs | |
| State | Published - 2017 Nov 1 |
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Keywords
- Flavan 3-ols
- Hindlimb suspension
- p70S6 kinase
- Ubiquitin ligase
ASJC Scopus subject areas
- Biochemistry
- Ageing
- Molecular Biology
- Genetics
- Endocrinology
- Cell Biology
Cite this
Flavan 3-ol delays the progression of disuse atrophy induced by hindlimb suspension in mice. / Ito, Mao; Kudo, Naoto; Miyake, Yuji; Imai, Tatsuya; Unno, Tomoki; Yamashita, Yoko; Hirota, Yoshihisa; Ashida, Hitoshi; Osakabe, Naomi.
In: Experimental Gerontology, Vol. 98, 01.11.2017, p. 120-123.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Flavan 3-ol delays the progression of disuse atrophy induced by hindlimb suspension in mice
AU - Ito,Mao
AU - Kudo,Naoto
AU - Miyake,Yuji
AU - Imai,Tatsuya
AU - Unno,Tomoki
AU - Yamashita,Yoko
AU - Hirota,Yoshihisa
AU - Ashida,Hitoshi
AU - Osakabe,Naomi
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Periods of skeletal muscle disuse, for example due to a sedentary lifestyle or bed rest, are associated with aging and can lead to muscle atrophy. We previously found that the flavan 3-ol fraction derived from cocoa (FL) enhanced energy expenditure with metabolic changes in skeletal muscle. In the present study, we examined the effect of FL on disuse muscle atrophy induced by hindlimb suspension in mice. Male C57BL/6J mice were assigned to four groups as follows: unsuspended-vehicle, unsuspended-FL, suspended-vehicle, and suspended-FL. Mice in the vehicle treatment groups were administered distilled water and those in the FL treatment groups were dosed with FL (50 mg/kg/day) for 2 weeks. The weights of the gastrocnemius (GC), tibialis anterior (TA), and soleus (SOL), but not the extensor digitorum longus (EDL), decreased significantly in mice with hindlimb suspension (− 11.8%, − 16.5%, and − 41.0%, respectively). This reduction in GC, TA, and SOL mass was inhibited by FL (− 5.3%, + 2.0%, and − 16.6%, respectively). The FL increased the EDL weight > 20% with or without hindlimb suspension. The protein level of the ubiquitin ligase, muscle ring finger-1, in the SOL was significantly increased by hindlimb suspension, but inhibited by treatment with FL. Protein expression of p70S6 kinase in the SOL was significantly decreased by hindlimb suspension, and FL treatment inhibited this change. These results suggested that FL delayed disuse muscle atrophy by metabolic alteration.
AB - Periods of skeletal muscle disuse, for example due to a sedentary lifestyle or bed rest, are associated with aging and can lead to muscle atrophy. We previously found that the flavan 3-ol fraction derived from cocoa (FL) enhanced energy expenditure with metabolic changes in skeletal muscle. In the present study, we examined the effect of FL on disuse muscle atrophy induced by hindlimb suspension in mice. Male C57BL/6J mice were assigned to four groups as follows: unsuspended-vehicle, unsuspended-FL, suspended-vehicle, and suspended-FL. Mice in the vehicle treatment groups were administered distilled water and those in the FL treatment groups were dosed with FL (50 mg/kg/day) for 2 weeks. The weights of the gastrocnemius (GC), tibialis anterior (TA), and soleus (SOL), but not the extensor digitorum longus (EDL), decreased significantly in mice with hindlimb suspension (− 11.8%, − 16.5%, and − 41.0%, respectively). This reduction in GC, TA, and SOL mass was inhibited by FL (− 5.3%, + 2.0%, and − 16.6%, respectively). The FL increased the EDL weight > 20% with or without hindlimb suspension. The protein level of the ubiquitin ligase, muscle ring finger-1, in the SOL was significantly increased by hindlimb suspension, but inhibited by treatment with FL. Protein expression of p70S6 kinase in the SOL was significantly decreased by hindlimb suspension, and FL treatment inhibited this change. These results suggested that FL delayed disuse muscle atrophy by metabolic alteration.
KW - Flavan 3-ols
KW - Hindlimb suspension
KW - p70S6 kinase
KW - Ubiquitin ligase
UR - http://www.scopus.com/inward/record.url?scp=85027534226&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027534226&partnerID=8YFLogxK
U2 - 10.1016/j.exger.2017.07.010
DO - 10.1016/j.exger.2017.07.010
M3 - Article
VL - 98
SP - 120
EP - 123
JO - Experimental Gerontology
T2 - Experimental Gerontology
JF - Experimental Gerontology
SN - 0531-5565
ER -