Flavan 3-ol delays the progression of disuse atrophy induced by hindlimb suspension in mice

Mao Ito, Naoto Kudo, Yuji Miyake, Tatsuya Imai, Tomoki Unno, Yoko Yamashita, Yoshihisa Hirota, Hitoshi Ashida, Naomi Osakabe

Research output: Contribution to journalArticle

Abstract

Periods of skeletal muscle disuse, for example due to a sedentary lifestyle or bed rest, are associated with aging and can lead to muscle atrophy. We previously found that the flavan 3-ol fraction derived from cocoa (FL) enhanced energy expenditure with metabolic changes in skeletal muscle. In the present study, we examined the effect of FL on disuse muscle atrophy induced by hindlimb suspension in mice. Male C57BL/6J mice were assigned to four groups as follows: unsuspended-vehicle, unsuspended-FL, suspended-vehicle, and suspended-FL. Mice in the vehicle treatment groups were administered distilled water and those in the FL treatment groups were dosed with FL (50 mg/kg/day) for 2 weeks. The weights of the gastrocnemius (GC), tibialis anterior (TA), and soleus (SOL), but not the extensor digitorum longus (EDL), decreased significantly in mice with hindlimb suspension (− 11.8%, − 16.5%, and − 41.0%, respectively). This reduction in GC, TA, and SOL mass was inhibited by FL (− 5.3%, + 2.0%, and − 16.6%, respectively). The FL increased the EDL weight > 20% with or without hindlimb suspension. The protein level of the ubiquitin ligase, muscle ring finger-1, in the SOL was significantly increased by hindlimb suspension, but inhibited by treatment with FL. Protein expression of p70S6 kinase in the SOL was significantly decreased by hindlimb suspension, and FL treatment inhibited this change. These results suggested that FL delayed disuse muscle atrophy by metabolic alteration.

LanguageEnglish
Pages120-123
Number of pages4
JournalExperimental Gerontology
Volume98
DOIs
StatePublished - 2017 Nov 1

Fingerprint

Muscle
Suspensions
Cocoa
Ligases
Ubiquitin
flavan-3-ol
Proteins
Phosphotransferases
Aging of materials
Water

Keywords

  • Flavan 3-ols
  • Hindlimb suspension
  • p70S6 kinase
  • Ubiquitin ligase

ASJC Scopus subject areas

  • Biochemistry
  • Ageing
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Flavan 3-ol delays the progression of disuse atrophy induced by hindlimb suspension in mice. / Ito, Mao; Kudo, Naoto; Miyake, Yuji; Imai, Tatsuya; Unno, Tomoki; Yamashita, Yoko; Hirota, Yoshihisa; Ashida, Hitoshi; Osakabe, Naomi.

In: Experimental Gerontology, Vol. 98, 01.11.2017, p. 120-123.

Research output: Contribution to journalArticle

Ito M, Kudo N, Miyake Y, Imai T, Unno T, Yamashita Y et al. Flavan 3-ol delays the progression of disuse atrophy induced by hindlimb suspension in mice. Experimental Gerontology. 2017 Nov 1;98:120-123. Available from, DOI: 10.1016/j.exger.2017.07.010
Ito, Mao ; Kudo, Naoto ; Miyake, Yuji ; Imai, Tatsuya ; Unno, Tomoki ; Yamashita, Yoko ; Hirota, Yoshihisa ; Ashida, Hitoshi ; Osakabe, Naomi. / Flavan 3-ol delays the progression of disuse atrophy induced by hindlimb suspension in mice. In: Experimental Gerontology. 2017 ; Vol. 98. pp. 120-123
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