Flexural rigidity of a single microtubule

Toru Takasone; Saulius Juodkazis; Yuji Kawagishi; Akira Yamaguchi; Shigeki Matsuo; Hitoshi Sakakibara; Haruto Nakayama; Hiroaki Misawa

doi:10.1143/jjap.41.3015

Flexural rigidity of a single microtubule

Toru Takasone, Saulius Juodkazis, Yuji Kawagishi, Akira Yamaguchi, Shigeki Matsuo, Hitoshi Sakakibara, Haruto Nakayama, Hiroaki Misawa

Research output: Contribution to journal › Article › peer-review

66 Citations (Scopus)

Abstract

Microtubules, which are flexible biopolymers, can be used for nanotechnology applications (e.g., nano-actuator) as they have a rigidity similar to that of plexyglass and other plastic materials. The flexural rigidity, or bending stiffness, of nucrotubules was measured using a laser trapping technique and dark-field microscopy. One end of a microtubule rod was chemically bound to a glass microsphere, while the other end was bound to a silica glass substrate. Then, the microsphere was lasertrapped and manipulated to exert three different deformation modes on the microtubule. The values of flexural rigidity for these deformations were between 10^-25 and 10^-23 Nm² as measured for the 5-25 μm length microtubules. The origin of the length dependence of the flexural rigidity of microtubules is discussed.

Original language	English
Pages (from-to)	3015-3019
Number of pages	5
Journal	Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume	41
Issue number	5 A
DOIs	https://doi.org/10.1143/jjap.41.3015
Publication status	Published - 2002 May
Externally published	Yes

Keywords

Flexural rigidity
Laser manipulation
Laser trapping
Laser tweezers
Microtubule-associated proteins
Radiation force

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)

Access to Document

10.1143/jjap.41.3015

Cite this

@article{9745676f68f440608d51431683d2d94d,

title = "Flexural rigidity of a single microtubule",

abstract = "Microtubules, which are flexible biopolymers, can be used for nanotechnology applications (e.g., nano-actuator) as they have a rigidity similar to that of plexyglass and other plastic materials. The flexural rigidity, or bending stiffness, of nucrotubules was measured using a laser trapping technique and dark-field microscopy. One end of a microtubule rod was chemically bound to a glass microsphere, while the other end was bound to a silica glass substrate. Then, the microsphere was lasertrapped and manipulated to exert three different deformation modes on the microtubule. The values of flexural rigidity for these deformations were between 10-25 and 10-23 Nm2 as measured for the 5-25 μm length microtubules. The origin of the length dependence of the flexural rigidity of microtubules is discussed.",

keywords = "Flexural rigidity, Laser manipulation, Laser trapping, Laser tweezers, Microtubule-associated proteins, Radiation force",

author = "Toru Takasone and Saulius Juodkazis and Yuji Kawagishi and Akira Yamaguchi and Shigeki Matsuo and Hitoshi Sakakibara and Haruto Nakayama and Hiroaki Misawa",

year = "2002",

month = may,

doi = "10.1143/jjap.41.3015",

language = "English",

volume = "41",

pages = "3015--3019",

journal = "Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes",

issn = "0021-4922",

publisher = "Japan Society of Applied Physics",

number = "5 A",

}

TY - JOUR

T1 - Flexural rigidity of a single microtubule

AU - Takasone, Toru

AU - Juodkazis, Saulius

AU - Kawagishi, Yuji

AU - Yamaguchi, Akira

AU - Matsuo, Shigeki

AU - Sakakibara, Hitoshi

AU - Nakayama, Haruto

AU - Misawa, Hiroaki

PY - 2002/5

Y1 - 2002/5

N2 - Microtubules, which are flexible biopolymers, can be used for nanotechnology applications (e.g., nano-actuator) as they have a rigidity similar to that of plexyglass and other plastic materials. The flexural rigidity, or bending stiffness, of nucrotubules was measured using a laser trapping technique and dark-field microscopy. One end of a microtubule rod was chemically bound to a glass microsphere, while the other end was bound to a silica glass substrate. Then, the microsphere was lasertrapped and manipulated to exert three different deformation modes on the microtubule. The values of flexural rigidity for these deformations were between 10-25 and 10-23 Nm2 as measured for the 5-25 μm length microtubules. The origin of the length dependence of the flexural rigidity of microtubules is discussed.

AB - Microtubules, which are flexible biopolymers, can be used for nanotechnology applications (e.g., nano-actuator) as they have a rigidity similar to that of plexyglass and other plastic materials. The flexural rigidity, or bending stiffness, of nucrotubules was measured using a laser trapping technique and dark-field microscopy. One end of a microtubule rod was chemically bound to a glass microsphere, while the other end was bound to a silica glass substrate. Then, the microsphere was lasertrapped and manipulated to exert three different deformation modes on the microtubule. The values of flexural rigidity for these deformations were between 10-25 and 10-23 Nm2 as measured for the 5-25 μm length microtubules. The origin of the length dependence of the flexural rigidity of microtubules is discussed.

KW - Flexural rigidity

KW - Laser manipulation

KW - Laser trapping

KW - Laser tweezers

KW - Microtubule-associated proteins

KW - Radiation force

UR - http://www.scopus.com/inward/record.url?scp=0036578222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036578222&partnerID=8YFLogxK

U2 - 10.1143/jjap.41.3015

DO - 10.1143/jjap.41.3015

M3 - Article

AN - SCOPUS:0036578222

SN - 0021-4922

VL - 41

SP - 3015

EP - 3019

JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

IS - 5 A

ER -

Flexural rigidity of a single microtubule

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this