Fluorometric bio-sniffer (gas phase biosensor) for breath acetone as a volatile product of lipid metabolism

Po Jen Chien, Ming Ye, Takuma Suzuki, Koji Toma, Takahiro Arakawa, Kohji Mitsubayashi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A highly sensitive acetone bio-sniffer (biochemical gas sensor) for assessment of lipid metabolism utilizing reverse reaction of secondary alcohol dehydrogenase was fabricated and demonstrated. This bio-sniffer consisted of a NADH fluorescence detection system, a flow-cell section, and an enzyme-immobilized membrane. This bio-sniffer presented rapid response, highly sensitivity and selectivity. The analyses of breath acetone concentrations during fasting and aerobic exercise were also demonstrated. The results showed that the breath acetone concentration would increase during long-time fasting and aerobic exercise. This acetone bio-sniffer provides a new kind of analytical tool for non-invasive evaluation of human lipid metabolism and it is also expected to use for the clinical applications such as monitoring the progression of diabetes.

Original languageEnglish
Title of host publicationChemical Sensors 12
Subtitle of host publicationChemical and Biological Sensors and Analytical Systems
EditorsM. Carter, Y. Shimizu, W. Y. Lee, T. Yasukawa, R. Mukundan, A. L. Simonian, L. A. Nagahara, O. Niwa, B. A. Chin
PublisherElectrochemical Society Inc.
Pages47-51
Number of pages5
Edition16
ISBN (Electronic)9781607687337, 9781607687672, 9781607687689, 9781607687696, 9781607687702, 9781607687719, 9781607687726, 9781607687733, 9781607687740, 9781607687757, 9781607687771, 9781607687788, 9781607687795, 9781607687801, 9781607687818, 9781607687825, 9781607687832, 9781607687849, 9781607687856, 9781607687863, 9781607687887, 9781607687894, 9781607687900, 9781607687917, 9781607687924, 9781607687931, 9781607687948, 9781607687955, 9781607687962, 9781607687979, 9781607687986, 9781607687993, 9781607688006, 9781607688013, 9781607688020, 9781607688037
DOIs
Publication statusPublished - 2016
Externally publishedYes
EventSymposium on Chemical Sensors 12: Chemical and Biological Sensors and Analytical Systems - PRiME 2016/230th ECS Meeting - Honolulu, United States
Duration: 2016 Oct 22016 Oct 7

Publication series

NameECS Transactions
Number16
Volume75
ISSN (Print)1938-6737
ISSN (Electronic)1938-5862

Conference

ConferenceSymposium on Chemical Sensors 12: Chemical and Biological Sensors and Analytical Systems - PRiME 2016/230th ECS Meeting
Country/TerritoryUnited States
CityHonolulu
Period16/10/216/10/7

ASJC Scopus subject areas

  • Engineering(all)

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