Fluorometric Biosniffer Camera "sniff-Cam" for Direct Imaging of Gaseous Ethanol in Breath and Transdermal Vapor

Takahiro Arakawa, Toshiyuki Sato, Kenta Iitani, Koji Toma, Kohji Mitsubayashi

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Various volatile organic compounds can be found in human transpiration, breath and body odor. In this paper, a novel two-dimensional fluorometric imaging system, known as a "sniffer-cam" for ethanol vapor released from human breath and palm skin was constructed and validated. This imaging system measures ethanol vapor concentrations as intensities of fluorescence through an enzymatic reaction induced by alcohol dehydrogenase (ADH). The imaging system consisted of multiple ultraviolet light emitting diode (UV-LED) excitation sheet, an ADH enzyme immobilized mesh substrate and a high-sensitive CCD camera. This imaging system uses ADH for recognition of ethanol vapor. It measures ethanol vapor by measuring fluorescence of nicotinamide adenine dinucleotide (NADH), which is produced by an enzymatic reaction on the mesh. This NADH fluorometric imaging system achieved the two-dimensional real-time imaging of ethanol vapor distribution (0.5-200 ppm). The system showed rapid and accurate responses and a visible measurement, which could lead to an analysis of metabolism function at real time in the near future.

Original languageEnglish
Pages (from-to)4495-4501
Number of pages7
JournalAnalytical Chemistry
Volume89
Issue number8
DOIs
Publication statusPublished - 2017 Apr 18
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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