Fluorometric imaging system 'sniffer-cam' for human breath and skin gas in evaluation of alcohol metabolism

Takahiro Arakawa, Kenta Iitani, Toshiyuki Sato, Munire Naisierding, Koji Toma, Kohji Mitsubayashi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Various volatile organic compounds can be found in human transpiration, breath and body odour. Therefore, a novel two-dimensional fluorometric imaging system, known as a 'sniffer-cam' for gaseous ethanol emissions from human breath and palm skin was constructed and validated. This imaging system measures ethanol vapour concentrations as intensities of fluorescence through an enzymatic reaction induced by alcohol dehydrogenase (ADH). The imaging system consisted of multi UV-LED excitation sheet and a high-sensitive CCD camera. This imaging system uses ADH for recognition of ethanol vapour. It measures ethanol vapour by measuring fluorescence of NADH, which is produced by an enzymatic reaction on the mesh. This NADH fluorometric imaging system achieved the two-dimensional real-time imaging of ethanol vapor distribution (0.5-200 ppm). The system showed a rapidly and accurately responses and a visible measurement, which could lead an analysis to metabolism function at real time.

Original languageEnglish
Title of host publication2016 International Conference on Optical MEMS and Nanophotonics, OMN 2016 - Proceedings
PublisherIEEE Computer Society
ISBN (Electronic)9781509010356
DOIs
Publication statusPublished - 2016 Sep 13
Externally publishedYes
Event21st International Conference on Optical MEMS and Nanophotonics, OMN 2016 - Singapore, Singapore
Duration: 2016 Jul 312016 Aug 4

Publication series

NameInternational Conference on Optical MEMS and Nanophotonics
Volume2016-September
ISSN (Print)2160-5033
ISSN (Electronic)2160-5041

Conference

Conference21st International Conference on Optical MEMS and Nanophotonics, OMN 2016
Country/TerritorySingapore
CitySingapore
Period16/7/3116/8/4

Keywords

  • enzyme
  • fluorometric imaging
  • human breath
  • NADH
  • skin gas
  • transdermal ethanol

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials

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