Functional analysis of the thermophilic denitrifying bacterium Geobacillus sp. strain TDN01 in continuous culture

Kota Nara, Kenichi Iwata, Toru Matsui, Toshiya Shigeno, Toshio Omori

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The thermophilic denitrifying bacterium Geobacillus sp. strain TDN01 was examined to determine the effects of nitrogen and carbon sources and nitrate and nitrite concentrations on denitrification in a batch culture. The specific nitrate removal rate was 12 times higher with ammonia than without ammonia. The consumption rates of nitrate and succinate were proportional. Furthermore, the growth rates with 120 and 150 mM nitrate were only slightly lower than those with 60 mM and did not cause notable growth inhibition. Denitrification ability in continuous culture was analyzed based on the data for batch culture. The maximum specific growth rate μmax and substrate saturation constant KS in the Monod equation were determined by gradually changing the dilution rate. The maximum denitrification rate was six times higher than that of mesophilic bacteria.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalJournal of General and Applied Microbiology
Issue number2
Publication statusPublished - 2009 Jun 29



  • Continuous culture
  • Denitrification
  • Geobacillus
  • Nitrate
  • Nitrite
  • Thermophilic bacteria

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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