Gene cloning of the maoA gene and overproduction of a soluble monoamine oxidase from Klebsiella aerogenes

Hiroyuki Sugino, Kaname Ishibashi, Masashi Sakaue, Mitsuo Yamashita, Yoshikatsu Murooka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We cloned the structural gene for monoamine oxidase (maoA) from Klebsiella aerogenes into a pKI212 vector in an maoA mutant strain of K. aerogenes. Deletion analysis and complementation tests of the recombinant plasmid showed that the maoA gene was located entirely within a 4.1-kb segment. In an maoA mutant strain harbouring the cloned maoA gene, synthesis of monoamine oxidase was induced by addition of tyramine and related compounds. Transfer of a plasmid containing the maoA gene into a monoamine oxidase-producing strain of K. aerogenes W70 resulted in about a 30- to 40-fold increase in total production of the enzyme. When cells of K. aerogenes carrying the plasmid containing the maoA gene were grown with tyramine, more than 85% of the monoamine oxidase was produced in soluble form, whereas the parent strain W70 produced most monoamine oxidase as the membrane-bound form.

Original languageEnglish
Pages (from-to)606-610
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume35
Issue number5
DOIs
Publication statusPublished - 1991 Aug
Externally publishedYes

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Cloning
Monoamine Oxidase
Genes
Tyramine
Plasmids
Oxidoreductases
Enzymes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology
  • Bioengineering
  • Biotechnology

Cite this

Gene cloning of the maoA gene and overproduction of a soluble monoamine oxidase from Klebsiella aerogenes. / Sugino, Hiroyuki; Ishibashi, Kaname; Sakaue, Masashi; Yamashita, Mitsuo; Murooka, Yoshikatsu.

In: Applied Microbiology and Biotechnology, Vol. 35, No. 5, 08.1991, p. 606-610.

Research output: Contribution to journalArticle

Sugino, Hiroyuki ; Ishibashi, Kaname ; Sakaue, Masashi ; Yamashita, Mitsuo ; Murooka, Yoshikatsu. / Gene cloning of the maoA gene and overproduction of a soluble monoamine oxidase from Klebsiella aerogenes. In: Applied Microbiology and Biotechnology. 1991 ; Vol. 35, No. 5. pp. 606-610.
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