TY - JOUR
T1 - Generation of novel genetically modified rats to reveal the molecular mechanisms of vitamin D actions
AU - Nishikawa, Miyu
AU - Yasuda, Kaori
AU - Takamatsu, Masashi
AU - Abe, Keisuke
AU - Okamoto, Kairi
AU - Horibe, Kyohei
AU - Mano, Hiroki
AU - Nakagawa, Kimie
AU - Tsugawa, Naoko
AU - Hirota, Yoshihisa
AU - Horie, Tetsuhiro
AU - Hinoi, Eiichi
AU - Okano, Toshio
AU - Ikushiro, Shinichi
AU - Sakaki, Toshiyuki
N1 - Funding Information:
We express our gratitude to Dr. Tatsuo Suda (Saitama Medical University, Saitama, Japan) for his kind advice and scientific discussions. This work was supported by Grants-in-Aid from the Japan Society for the Promotion of Science (No. 16H04912 to T.S.).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Recent studies have suggested that vitamin D activities involve vitamin D receptor (VDR)-dependent and VDR-independent effects of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and 25-hydroxyvitamin D3 (25(OH)D3) and ligand-independent effects of the VDR. Here, we describe a novel in vivo system using genetically modified rats deficient in the Cyp27b1 or Vdr genes. Type II rickets model rats with a mutant Vdr (R270L), which recognizes 1,25(OH)2D3 with an affinity equivalent to that for 25(OH)D3, were also generated. Although Cyp27b1-knockout (KO), Vdr-KO, and Vdr (R270L) rats each showed rickets symptoms, including abnormal bone formation, they were significantly different from each other. Administration of 25(OH)D3 reversed rickets symptoms in Cyp27b1-KO and Vdr (R270L) rats. Interestingly, 1,25(OH)2D3 was synthesized in Cyp27b1-KO rats, probably by Cyp27a1. In contrast, the effects of 25(OH)D3 on Vdr (R270L) rats strongly suggested a direct action of 25(OH)D3 via VDR-genomic pathways. These results convincingly suggest the usefulness of our in vivo system.
AB - Recent studies have suggested that vitamin D activities involve vitamin D receptor (VDR)-dependent and VDR-independent effects of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and 25-hydroxyvitamin D3 (25(OH)D3) and ligand-independent effects of the VDR. Here, we describe a novel in vivo system using genetically modified rats deficient in the Cyp27b1 or Vdr genes. Type II rickets model rats with a mutant Vdr (R270L), which recognizes 1,25(OH)2D3 with an affinity equivalent to that for 25(OH)D3, were also generated. Although Cyp27b1-knockout (KO), Vdr-KO, and Vdr (R270L) rats each showed rickets symptoms, including abnormal bone formation, they were significantly different from each other. Administration of 25(OH)D3 reversed rickets symptoms in Cyp27b1-KO and Vdr (R270L) rats. Interestingly, 1,25(OH)2D3 was synthesized in Cyp27b1-KO rats, probably by Cyp27a1. In contrast, the effects of 25(OH)D3 on Vdr (R270L) rats strongly suggested a direct action of 25(OH)D3 via VDR-genomic pathways. These results convincingly suggest the usefulness of our in vivo system.
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U2 - 10.1038/s41598-020-62048-1
DO - 10.1038/s41598-020-62048-1
M3 - Article
C2 - 32231239
AN - SCOPUS:85082556179
VL - 10
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 5677
ER -