Genetic and Physiological Characteristics of a Novel Marine Propylene-Assimilating Halieaceae Bacterium Isolated from Seawater and the Diversity of Its Alkene and Epoxide Metabolism Genes

Toshihiro Suzuki, Tomoki Yazawa, Naonori Morishita, Akihiko Maruyama, Hiroyuki Fuse

Research output: Contribution to journalArticle

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Abstract

The Gram-negative marine propylene-assimilating bacterium, strain PE-TB08W, was isolated from surface seawater. A structural gene analysis using the 16S rRNA gene showed 96, 94, and 95% similarities to Halioglobus species, Haliea sp. ETY-M, and Haliea sp. ETY-NAG, respectively. A phylogenetic tree analysis showed that strain PE-TB08W belonged to the EG19 (Chromatocurvus)-Congregibacter-Haliea cluster within the Halieaceae (formerly Alteromonadaceae) family. Thus, strain PE-TB08W was characterized as a newly isolated Halieaceae bacterium; we suggest that this strain belongs to a new genus. Other bacterial characteristics were investigated and revealed that strain PE-TB08W assimilated propylene, n-butane, 1-butene, propanol, and 1-butanol (C3 and C4 gaseous hydrocarbons and primary alcohols), but not various other alcohols, including methane, ethane, ethylene, propane, and i-butane. The putative alkene monooxygenase (amo) gene in this strain was a soluble methane monooxygenase-type (sMMO) gene that is ubiquitous in alkene-assimilating bacteria for the initial oxidation of alkenes. In addition, two epoxide carboxylase systems containing epoxyalkane, the co-enzyme M transferase (EaCoMT) gene, and the co-enzyme M biosynthesis gene, were found in the upstream region of the sMMO gene cluster. Both of these genes were similar to those in Xanthobacter autotrophicus Py2 and were inductively expressed by propylene. These results have a significant impact on the genetic relationship between terrestrial and marine alkene-assimilating bacteria.

Original languageEnglish
Pages (from-to)33-42
Number of pages10
JournalMicrobes and environments
Volume34
Issue number1
DOIs
Publication statusPublished - 2019 Mar 30

Fingerprint

propylene
alkenes
alkene
epoxides
seawater
metabolism
bacterium
gene
bacteria
genes
coenzymes
Xanthobacter autotrophicus
Alteromonadaceae
methane
alcohols
1-propanol
alcohol
ethane
propane
structural genes

Keywords

  • alkene monooxygenase
  • EaCoMT
  • Halieaceae family
  • marine bacterium
  • propylene assimilation

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

Cite this

Genetic and Physiological Characteristics of a Novel Marine Propylene-Assimilating Halieaceae Bacterium Isolated from Seawater and the Diversity of Its Alkene and Epoxide Metabolism Genes. / Suzuki, Toshihiro; Yazawa, Tomoki; Morishita, Naonori; Maruyama, Akihiko; Fuse, Hiroyuki.

In: Microbes and environments, Vol. 34, No. 1, 30.03.2019, p. 33-42.

Research output: Contribution to journalArticle

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