Genetic characterisation of genes involved in the upper pathway of carbazole metabolism from the putative Kordiimonas sp

Yoshihiko Ito, Rintaro Maeda, Kenichi Iwata, Toshio Omori

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The car genes from a carbazole (CAR)-degrading bacterium, Kordiimonas sp. OC9, were functionally and transcriptionally analysed. The enzymatic activity for the protein coded by carBaBb using pBOC93 (carAaAcBa), pBOC93-2 (carAaAcBb), and pBOC94 (carAaAcBaBb) was confirmed. Resting cells using Escherichia coli harbouring pBOC95 (carAaAcBaBbC) revealed the function of the carC gene product in the conversion of CAR to anthranilic acid by expressing it with CarAaAcBaBb. The pathway of CAR metabolism to anthranilic acid in marine CAR-degraders was elucidated. Transcriptional analysis using RT-PCR revealed that car genes are related to CAR degradation in response to CAR exposure in strain OC9. RT-PCR analysis of the operon structure showed that the car gene cluster of strain OC9 has two distinct operons in one car gene cluster. The localisation of the car gene cluster of strain OC9 was also determined.

Original languageEnglish
Pages (from-to)1859-1864
Number of pages6
JournalBiotechnology Letters
Volume33
Issue number9
DOIs
Publication statusPublished - 2011 Sep

Fingerprint

Metabolism
Genes
Railroad cars
Acids
Escherichia coli
carbazole
Bacteria
Proteins
Degradation

Keywords

  • Bioremediation
  • Carbazole
  • Kordiimonas
  • Localisation of gene cluster
  • Transcriptional analysis

ASJC Scopus subject areas

  • Biotechnology

Cite this

Genetic characterisation of genes involved in the upper pathway of carbazole metabolism from the putative Kordiimonas sp. / Ito, Yoshihiko; Maeda, Rintaro; Iwata, Kenichi; Omori, Toshio.

In: Biotechnology Letters, Vol. 33, No. 9, 09.2011, p. 1859-1864.

Research output: Contribution to journalArticle

Ito, Yoshihiko ; Maeda, Rintaro ; Iwata, Kenichi ; Omori, Toshio. / Genetic characterisation of genes involved in the upper pathway of carbazole metabolism from the putative Kordiimonas sp. In: Biotechnology Letters. 2011 ; Vol. 33, No. 9. pp. 1859-1864.
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