Genomic structure of the actin-encoding gene of Pneumocystis carinii

Yasushi Miyahira, Yoshiki Hiraoka, Naoki Komatsu, Tsutomu Takeuchi, Sadakazu Aiso

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have isolated both the genomic and cDNA clones of the actin gene of Pneumocystis carinii and analyzed its genomic structure. The gene consists of eight exons. The introns are uniformly short in size (43-49 nucleotides). Multiple poly(A) sites of the actin mRNA are located in a highly AT-rich portion of the 3′-flanking region of this gene, although the canonical poly(A) signal, AATAAA, is not found in the region. Transcription initiation sites have been determined by the primer extension method. A TATA-box-like sequence is located 39-bp upstream of the 5′-most major initiation site.

Original languageEnglish
Pages (from-to)289-295
Number of pages7
JournalParasitology International
Volume46
Issue number4
DOIs
Publication statusPublished - 1997 Dec

Keywords

  • Actin
  • Genomic structure
  • Nucleotide sequence
  • Opportunistic infection
  • Pathogen
  • Pneumocystis carinii

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Genomic structure of the actin-encoding gene of Pneumocystis carinii'. Together they form a unique fingerprint.

  • Cite this