Glucose-driven chemo-mechanical autonomous drug-release system with multi-enzymatic amplification toward feedback control of blood glucose in diabetes

Munkhbayar Munkhjargal, Kohdai Hatayama, Yuki Matsuura, Koji Toma, Takahiro Arakawa, Kohji Mitsubayashi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A second-generation novel chemo-mechanical autonomous drug release system, incorporating various improvements over our first-generation system, was fabricated and evaluated. Enhanced oxygen uptake by the enzyme membrane of the organic engine was facilitated by optimizing the quantity of enzyme immobilizer, PVA-SbQ, and by hydrophobizing the membrane surface. Various quantities of PVA-SbQ were evaluated in the organic engine by measuring the decompression rate, with 1.5mg/cm2 yielding optimum results. When fluororesin was used as a hydrophobizing coating, the time to reach the peak decompression rate was shortened 2.3-fold. The optimized elements of the system were evaluated as a unit, first in an open loop and then in a closed loop setting, using a mixture of glucose solution (25mmol/L), ATP and MgCI2 with glucose hexokinase enzyme (HK) as a glucose reducer. In conclusion, feedback-control of physiologically relevant glucose concentration was demonstrated by the second-generation drug release system without any requirement for external energy.

Original languageEnglish
Pages (from-to)315-320
Number of pages6
JournalBiosensors and Bioelectronics
Volume67
DOIs
Publication statusPublished - 2015 Mar 1
Externally publishedYes

Keywords

  • Artificial pancreas
  • Chemo-mechanical
  • Enzyme membrane
  • Hexokinase

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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