Gravitaxis in Chlamydomonas reinhardtii Studied with Novel Mutants

Kenjiro Yoshimura, Yudo Matsuo, Ritsu Kamiya

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Many free-swimming unicellular organisms show negative gravitaxis, i.e. tend to swim upward, although their specific densities are higher than the medium density. To obtain clues to the mechanism of this behavior, we examined how a mutation in motility or behavior affects the gravitaxis in Chlamydomonas. A phototaxis mutant, ptx3, deficient in membrane excitability showed weakened gravitaxis, whereas another phototaxis mutant, ptx1, deficient in regulation of flagellar dominance displayed normal gravitaxis. Two mutants that swim backwards only, mbo1 and mbo2, did not show any clear gravitaxis. We also isolated two novel mutants deficient in gravitaxis, gtx1 and gtx2. These mutants displayed normal motility and physical characteristics of cell body as assessed by the behavior of anesthetized cells. However, these cells were found to have defects in physiological responses involving membrane excitation. These observations are consistent with the idea that the gravitaxis in Chlamydomonas involves a physiological signal transduction system, which is at least partially independent of the system used for phototaxis.

Original languageEnglish
Pages (from-to)1112-1118
Number of pages7
JournalPlant and Cell Physiology
Volume44
Issue number10
DOIs
Publication statusPublished - 2003 Oct
Externally publishedYes

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geotaxis
Chlamydomonas reinhardtii
mutants
phototaxis
Chlamydomonas
cells
physiological response
dominance (genetics)
signal transduction
mutation
organisms

Keywords

  • Chlamydomonas
  • Gravitaxis
  • Mutant
  • Phototaxis
  • Unicellular algae

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Gravitaxis in Chlamydomonas reinhardtii Studied with Novel Mutants. / Yoshimura, Kenjiro; Matsuo, Yudo; Kamiya, Ritsu.

In: Plant and Cell Physiology, Vol. 44, No. 10, 10.2003, p. 1112-1118.

Research output: Contribution to journalArticle

Yoshimura, Kenjiro ; Matsuo, Yudo ; Kamiya, Ritsu. / Gravitaxis in Chlamydomonas reinhardtii Studied with Novel Mutants. In: Plant and Cell Physiology. 2003 ; Vol. 44, No. 10. pp. 1112-1118.
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