Growth hormone-releasing factor (GRF) suppresses the in vitro proliferation of mammotrophs from the adult rat

Tadashi Shinkai, Hiroshi Ooka, Tetsuo Noumura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In order to examine the hypothalamic control of acidophilic proliferation, anterior pituitary cells from adult female rats were cultured with or without rat growth hormone-releasing factor fragment 1-29 (GRF-29). Changes in the numbers of mammotrophs and somatotrophs during culture were measured by immunocytochemical staining. The addition of GRF suppressed the increase in the number of mammotrophs even at the very low concentration of 10-12 M. The number of somatotrophs increased in the medium containing GRF. The increase in mammotroph number was blocked by cytosine arabinoside, a mitotic inhibitor. GRF had no effect on the in vitro proliferation of fibroblasts. These results indicate the important role of hypothalamic GRF in the differential growth and secretion of the acidophils in vivo.

Original languageEnglish
Pages (from-to)13-16
Number of pages4
JournalNeuroscience Letters
Volume123
Issue number1
DOIs
Publication statusPublished - 1991 Feb 11
Externally publishedYes

Keywords

  • Culture
  • GRF
  • Mammotroph
  • Rat
  • Somatotroph

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Growth hormone-releasing factor (GRF) suppresses the in vitro proliferation of mammotrophs from the adult rat. / Shinkai, Tadashi; Ooka, Hiroshi; Noumura, Tetsuo.

In: Neuroscience Letters, Vol. 123, No. 1, 11.02.1991, p. 13-16.

Research output: Contribution to journalArticle

Shinkai, Tadashi ; Ooka, Hiroshi ; Noumura, Tetsuo. / Growth hormone-releasing factor (GRF) suppresses the in vitro proliferation of mammotrophs from the adult rat. In: Neuroscience Letters. 1991 ; Vol. 123, No. 1. pp. 13-16.
@article{4577d6ede7ca48d0a07faf0dbdd9ca99,
title = "Growth hormone-releasing factor (GRF) suppresses the in vitro proliferation of mammotrophs from the adult rat",
abstract = "In order to examine the hypothalamic control of acidophilic proliferation, anterior pituitary cells from adult female rats were cultured with or without rat growth hormone-releasing factor fragment 1-29 (GRF-29). Changes in the numbers of mammotrophs and somatotrophs during culture were measured by immunocytochemical staining. The addition of GRF suppressed the increase in the number of mammotrophs even at the very low concentration of 10-12 M. The number of somatotrophs increased in the medium containing GRF. The increase in mammotroph number was blocked by cytosine arabinoside, a mitotic inhibitor. GRF had no effect on the in vitro proliferation of fibroblasts. These results indicate the important role of hypothalamic GRF in the differential growth and secretion of the acidophils in vivo.",
keywords = "Culture, GRF, Mammotroph, Rat, Somatotroph",
author = "Tadashi Shinkai and Hiroshi Ooka and Tetsuo Noumura",
year = "1991",
month = "2",
day = "11",
doi = "10.1016/0304-3940(91)90146-K",
language = "English",
volume = "123",
pages = "13--16",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Growth hormone-releasing factor (GRF) suppresses the in vitro proliferation of mammotrophs from the adult rat

AU - Shinkai, Tadashi

AU - Ooka, Hiroshi

AU - Noumura, Tetsuo

PY - 1991/2/11

Y1 - 1991/2/11

N2 - In order to examine the hypothalamic control of acidophilic proliferation, anterior pituitary cells from adult female rats were cultured with or without rat growth hormone-releasing factor fragment 1-29 (GRF-29). Changes in the numbers of mammotrophs and somatotrophs during culture were measured by immunocytochemical staining. The addition of GRF suppressed the increase in the number of mammotrophs even at the very low concentration of 10-12 M. The number of somatotrophs increased in the medium containing GRF. The increase in mammotroph number was blocked by cytosine arabinoside, a mitotic inhibitor. GRF had no effect on the in vitro proliferation of fibroblasts. These results indicate the important role of hypothalamic GRF in the differential growth and secretion of the acidophils in vivo.

AB - In order to examine the hypothalamic control of acidophilic proliferation, anterior pituitary cells from adult female rats were cultured with or without rat growth hormone-releasing factor fragment 1-29 (GRF-29). Changes in the numbers of mammotrophs and somatotrophs during culture were measured by immunocytochemical staining. The addition of GRF suppressed the increase in the number of mammotrophs even at the very low concentration of 10-12 M. The number of somatotrophs increased in the medium containing GRF. The increase in mammotroph number was blocked by cytosine arabinoside, a mitotic inhibitor. GRF had no effect on the in vitro proliferation of fibroblasts. These results indicate the important role of hypothalamic GRF in the differential growth and secretion of the acidophils in vivo.

KW - Culture

KW - GRF

KW - Mammotroph

KW - Rat

KW - Somatotroph

UR - http://www.scopus.com/inward/record.url?scp=0026034351&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026034351&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(91)90146-K

DO - 10.1016/0304-3940(91)90146-K

M3 - Article

C2 - 2062449

AN - SCOPUS:0026034351

VL - 123

SP - 13

EP - 16

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1

ER -