Growth hormone-releasing factor (GRF) suppresses the in vitro proliferation of mammotrophs from the adult rat

Tadashi Shinkai; Hiroshi Ooka; Tetsuo Noumura

doi:10.1016/0304-3940(91)90146-K

Growth hormone-releasing factor (GRF) suppresses the in vitro proliferation of mammotrophs from the adult rat

Tadashi Shinkai, Hiroshi Ooka, Tetsuo Noumura

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

In order to examine the hypothalamic control of acidophilic proliferation, anterior pituitary cells from adult female rats were cultured with or without rat growth hormone-releasing factor fragment 1-29 (GRF-29). Changes in the numbers of mammotrophs and somatotrophs during culture were measured by immunocytochemical staining. The addition of GRF suppressed the increase in the number of mammotrophs even at the very low concentration of 10^-12 M. The number of somatotrophs increased in the medium containing GRF. The increase in mammotroph number was blocked by cytosine arabinoside, a mitotic inhibitor. GRF had no effect on the in vitro proliferation of fibroblasts. These results indicate the important role of hypothalamic GRF in the differential growth and secretion of the acidophils in vivo.

Original language	English
Pages (from-to)	13-16
Number of pages	4
Journal	Neuroscience Letters
Volume	123
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(91)90146-K
Publication status	Published - 1991 Feb 11

Keywords

Culture
GRF
Mammotroph
Rat
Somatotroph

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(91)90146-K

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title = "Growth hormone-releasing factor (GRF) suppresses the in vitro proliferation of mammotrophs from the adult rat",

abstract = "In order to examine the hypothalamic control of acidophilic proliferation, anterior pituitary cells from adult female rats were cultured with or without rat growth hormone-releasing factor fragment 1-29 (GRF-29). Changes in the numbers of mammotrophs and somatotrophs during culture were measured by immunocytochemical staining. The addition of GRF suppressed the increase in the number of mammotrophs even at the very low concentration of 10-12 M. The number of somatotrophs increased in the medium containing GRF. The increase in mammotroph number was blocked by cytosine arabinoside, a mitotic inhibitor. GRF had no effect on the in vitro proliferation of fibroblasts. These results indicate the important role of hypothalamic GRF in the differential growth and secretion of the acidophils in vivo.",

keywords = "Culture, GRF, Mammotroph, Rat, Somatotroph",

author = "Tadashi Shinkai and Hiroshi Ooka and Tetsuo Noumura",

note = "Funding Information: We are grateful to Professor K. Wakabayashi and Dr. S. Tanaka of Gunma University for the supply of antibodies. This study was supported in part by a Grant-in-Aid 01740449 from the Ministry of Education, Science and Culture of Japan. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1991",

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doi = "10.1016/0304-3940(91)90146-K",

language = "English",

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AU - Shinkai, Tadashi

AU - Ooka, Hiroshi

AU - Noumura, Tetsuo

N1 - Funding Information: We are grateful to Professor K. Wakabayashi and Dr. S. Tanaka of Gunma University for the supply of antibodies. This study was supported in part by a Grant-in-Aid 01740449 from the Ministry of Education, Science and Culture of Japan. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1991/2/11

Y1 - 1991/2/11

N2 - In order to examine the hypothalamic control of acidophilic proliferation, anterior pituitary cells from adult female rats were cultured with or without rat growth hormone-releasing factor fragment 1-29 (GRF-29). Changes in the numbers of mammotrophs and somatotrophs during culture were measured by immunocytochemical staining. The addition of GRF suppressed the increase in the number of mammotrophs even at the very low concentration of 10-12 M. The number of somatotrophs increased in the medium containing GRF. The increase in mammotroph number was blocked by cytosine arabinoside, a mitotic inhibitor. GRF had no effect on the in vitro proliferation of fibroblasts. These results indicate the important role of hypothalamic GRF in the differential growth and secretion of the acidophils in vivo.

AB - In order to examine the hypothalamic control of acidophilic proliferation, anterior pituitary cells from adult female rats were cultured with or without rat growth hormone-releasing factor fragment 1-29 (GRF-29). Changes in the numbers of mammotrophs and somatotrophs during culture were measured by immunocytochemical staining. The addition of GRF suppressed the increase in the number of mammotrophs even at the very low concentration of 10-12 M. The number of somatotrophs increased in the medium containing GRF. The increase in mammotroph number was blocked by cytosine arabinoside, a mitotic inhibitor. GRF had no effect on the in vitro proliferation of fibroblasts. These results indicate the important role of hypothalamic GRF in the differential growth and secretion of the acidophils in vivo.

KW - Culture

KW - GRF

KW - Mammotroph

KW - Rat

KW - Somatotroph

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Growth hormone-releasing factor (GRF) suppresses the in vitro proliferation of mammotrophs from the adult rat

Abstract

Keywords

ASJC Scopus subject areas

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