High-speed single cell dispensing system

Tomohiro Kawahara, Tatsuhiko Hirano, Lin Feng, Huseyin Uvet, Masaya Hagiwara, Yoko Yamanishi, Fumihito Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we discuss the single cell dispensing system to increase both the speed and the success rate of single cell dispensing. Two pairs of capacitance sensors are placed in a biochip to detect the flow velocity of cells, and the air pressure is applied to eject cells by synchronizing the timing. Then, the system theoretically has a capability to eject 3 cells/sec with maximum flow velocity is 10 mm/sec. Finally, we succeeded in automatic dispensing of a single polystyrene bead (=100 μm) from a biochip to culture well atmosphere using developed cell ejection system with the success rate of 50 %. Furthermore, we also succeeded in single swine oocyte dispensing by developed system.

Original languageEnglish
Title of host publication2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
Pages472-474
Number of pages3
DOIs
Publication statusPublished - 2012
Externally publishedYes
Event22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation - Nagoya
Duration: 2011 Nov 62011 Nov 9

Other

Other22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation
CityNagoya
Period11/11/611/11/9

Fingerprint

Biochips
Flow velocity
Polystyrenes
Capacitance
Sensors
Air

ASJC Scopus subject areas

  • Artificial Intelligence
  • Mechanical Engineering

Cite this

Kawahara, T., Hirano, T., Feng, L., Uvet, H., Hagiwara, M., Yamanishi, Y., & Arai, F. (2012). High-speed single cell dispensing system. In 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation" (pp. 472-474). [6102235] https://doi.org/10.1109/MHS.2011.6102235

High-speed single cell dispensing system. / Kawahara, Tomohiro; Hirano, Tatsuhiko; Feng, Lin; Uvet, Huseyin; Hagiwara, Masaya; Yamanishi, Yoko; Arai, Fumihito.

2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. p. 472-474 6102235.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kawahara, T, Hirano, T, Feng, L, Uvet, H, Hagiwara, M, Yamanishi, Y & Arai, F 2012, High-speed single cell dispensing system. in 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"., 6102235, pp. 472-474, 22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation, Nagoya, 11/11/6. https://doi.org/10.1109/MHS.2011.6102235
Kawahara T, Hirano T, Feng L, Uvet H, Hagiwara M, Yamanishi Y et al. High-speed single cell dispensing system. In 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. p. 472-474. 6102235 https://doi.org/10.1109/MHS.2011.6102235
Kawahara, Tomohiro ; Hirano, Tatsuhiko ; Feng, Lin ; Uvet, Huseyin ; Hagiwara, Masaya ; Yamanishi, Yoko ; Arai, Fumihito. / High-speed single cell dispensing system. 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. pp. 472-474
@inproceedings{4cca7b4ab02d456fb7886388e10d924f,
title = "High-speed single cell dispensing system",
abstract = "In this paper, we discuss the single cell dispensing system to increase both the speed and the success rate of single cell dispensing. Two pairs of capacitance sensors are placed in a biochip to detect the flow velocity of cells, and the air pressure is applied to eject cells by synchronizing the timing. Then, the system theoretically has a capability to eject 3 cells/sec with maximum flow velocity is 10 mm/sec. Finally, we succeeded in automatic dispensing of a single polystyrene bead (=100 μm) from a biochip to culture well atmosphere using developed cell ejection system with the success rate of 50 {\%}. Furthermore, we also succeeded in single swine oocyte dispensing by developed system.",
author = "Tomohiro Kawahara and Tatsuhiko Hirano and Lin Feng and Huseyin Uvet and Masaya Hagiwara and Yoko Yamanishi and Fumihito Arai",
year = "2012",
doi = "10.1109/MHS.2011.6102235",
language = "English",
isbn = "9781457713613",
pages = "472--474",
booktitle = "2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on {"}COE for Education and Research of Micro-Nano Mechatronics{"}, Symposium on {"}Hyper Bio Assembler for 3D Cellular System Innovation{"}",

}

TY - GEN

T1 - High-speed single cell dispensing system

AU - Kawahara, Tomohiro

AU - Hirano, Tatsuhiko

AU - Feng, Lin

AU - Uvet, Huseyin

AU - Hagiwara, Masaya

AU - Yamanishi, Yoko

AU - Arai, Fumihito

PY - 2012

Y1 - 2012

N2 - In this paper, we discuss the single cell dispensing system to increase both the speed and the success rate of single cell dispensing. Two pairs of capacitance sensors are placed in a biochip to detect the flow velocity of cells, and the air pressure is applied to eject cells by synchronizing the timing. Then, the system theoretically has a capability to eject 3 cells/sec with maximum flow velocity is 10 mm/sec. Finally, we succeeded in automatic dispensing of a single polystyrene bead (=100 μm) from a biochip to culture well atmosphere using developed cell ejection system with the success rate of 50 %. Furthermore, we also succeeded in single swine oocyte dispensing by developed system.

AB - In this paper, we discuss the single cell dispensing system to increase both the speed and the success rate of single cell dispensing. Two pairs of capacitance sensors are placed in a biochip to detect the flow velocity of cells, and the air pressure is applied to eject cells by synchronizing the timing. Then, the system theoretically has a capability to eject 3 cells/sec with maximum flow velocity is 10 mm/sec. Finally, we succeeded in automatic dispensing of a single polystyrene bead (=100 μm) from a biochip to culture well atmosphere using developed cell ejection system with the success rate of 50 %. Furthermore, we also succeeded in single swine oocyte dispensing by developed system.

UR - http://www.scopus.com/inward/record.url?scp=84863315376&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863315376&partnerID=8YFLogxK

U2 - 10.1109/MHS.2011.6102235

DO - 10.1109/MHS.2011.6102235

M3 - Conference contribution

SN - 9781457713613

SP - 472

EP - 474

BT - 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"

ER -