High-speed single cell dispensing system

Tomohiro Kawahara, Tatsuhiko Hirano, Lin Feng, Huseyin Uvet, Masaya Hagiwara, Yoko Yamanishi, Fumihito Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we discuss the single cell dispensing system to increase both the speed and the success rate of single cell dispensing. Two pairs of capacitance sensors are placed in a biochip to detect the flow velocity of cells, and the air pressure is applied to eject cells by synchronizing the timing. Then, the system theoretically has a capability to eject 3 cells/sec with maximum flow velocity is 10 mm/sec. Finally, we succeeded in automatic dispensing of a single polystyrene bead (=100 μm) from a biochip to culture well atmosphere using developed cell ejection system with the success rate of 50 %. Furthermore, we also succeeded in single swine oocyte dispensing by developed system.

Original languageEnglish
Title of host publication2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
PublisherIEEE Computer Society
Pages472-474
Number of pages3
ISBN (Print)9781457713613
DOIs
Publication statusPublished - 2011 Jan 1
Event22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation - Nagoya, Japan
Duration: 2011 Nov 62011 Nov 9

Publication series

Name2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"

Conference

Conference22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation
CountryJapan
CityNagoya
Period11/11/611/11/9

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ASJC Scopus subject areas

  • Artificial Intelligence
  • Mechanical Engineering

Cite this

Kawahara, T., Hirano, T., Feng, L., Uvet, H., Hagiwara, M., Yamanishi, Y., & Arai, F. (2011). High-speed single cell dispensing system. In 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation" (pp. 472-474). [6102235] (2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"). IEEE Computer Society. https://doi.org/10.1109/MHS.2011.6102235