Highly integrated transparent microchannel chips for continuous-flow PCR

Kai Sun, Shigeki Matsuo, Hiroaki Misawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A transparent microchannel chip for continuous-flow polymerase chain reaction (PCR) was developed. The microchannel was fabricated on a cover glass substrate using standard photolithography and wet-etching techniques, and sealed by another cover glass substrate. A heat source consisting of two indium-tin-oxide (ITO) films was deposited on the etched substrate. An insulation layer of SiO2 was then deposited on the ITO heaters. Thin, patterned ITO films deposited on the insulation layer served as temperature sensors. Linear relationship between the thin ITO film sheet resistivity and the temperature was confirmed and calibrated. An amplication of a 97-bp segment of rat tongue cDNA was successfully performed with the chip.

Original languageEnglish
Title of host publication2003 Nanotechnology Conference and Trade Show - Nanotech 2003
EditorsM. Laudon, B. Romanowicz
Pages86-89
Number of pages4
Publication statusPublished - 2003 Dec 1
Externally publishedYes
Event2003 Nanotechnology Conference and Trade Show - Nanotech 2003 - San Francisco, CA, United States
Duration: 2003 Feb 232003 Feb 27

Publication series

Name2003 Nanotechnology Conference and Trade Show - Nanotech 2003
Volume1

Conference

Conference2003 Nanotechnology Conference and Trade Show - Nanotech 2003
CountryUnited States
CitySan Francisco, CA
Period03/2/2303/2/27

Keywords

  • Heater
  • ITO
  • Microchannel
  • PCR
  • Sensor

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Sun, K., Matsuo, S., & Misawa, H. (2003). Highly integrated transparent microchannel chips for continuous-flow PCR. In M. Laudon, & B. Romanowicz (Eds.), 2003 Nanotechnology Conference and Trade Show - Nanotech 2003 (pp. 86-89). (2003 Nanotechnology Conference and Trade Show - Nanotech 2003; Vol. 1).