Highly sensitive fluorometric imaging system 'sniff-CAM' for analysis of volatile organic compounds from skin and breath

Takahiro Arakawa, Kenta Iitanr, Naoki Mizukoshi, Toma Koji, Kohji Mitsubayashi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Various volatile organic compounds can be found in human transpiration, breath and body odor. Progress in the analysis of volatile organic compounds, such as those found in the breath and transdermal gas, creates an increasing potential for the application of this concept to the medical field. In this study, a novel two-dimensional fluorometric imaging system, known as a 'sniffer-cam' for ethanol vapor released from human breath and palm skin was constructed and validated. This imaging system measures ethanol vapor concentrations as intensities of fluorescence through an enzymatic reaction induced by alcohol dehydrogenase. The imaging system consisted of multiple ultra violet light emitting diode excitation sheet, an enzyme immobilized mesh substrate and a high-sensitive CCD camera. This imaging system uses alcohol dehydrogenase for recognition of ethanol vapor. It measures ethanol vapor by measuring fluorescence of nicotinamide adenine dinucleotide (NADH), which is produced by an enzymatic reaction on the mesh. The fluorescent properties of NADH (ex. 340 nm, fl. 490 nm), combining an excitation light source with a high-sensitivity camera allows us to detect and visualize NADH generated by the enzyme reaction when the ethanol gas is loaded. Based on this principle, we developed an imaging system that uses a fluorometric method for human breath and skin gas.

Original languageEnglish
Title of host publication2019 13th International Conference on Sensing Technology, ICST 2019
PublisherIEEE Computer Society
ISBN (Electronic)9781728146317
DOIs
Publication statusPublished - 2019 Dec
Externally publishedYes
Event13th International Conference on Sensing Technology, ICST 2019 - Sydney, Australia
Duration: 2019 Dec 22019 Dec 4

Publication series

NameProceedings of the International Conference on Sensing Technology, ICST
Volume2019-December
ISSN (Print)2156-8065
ISSN (Electronic)2156-8073

Conference

Conference13th International Conference on Sensing Technology, ICST 2019
Country/TerritoryAustralia
CitySydney
Period19/12/219/12/4

Keywords

  • Biosensor
  • Breath
  • Enzyme
  • Gas
  • Imaging
  • Skin

ASJC Scopus subject areas

  • Artificial Intelligence
  • Computer Science Applications
  • Signal Processing
  • Electrical and Electronic Engineering

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