Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats

Shinzo Kato, Hiromasa Ishii, Sadakazu Aiso, Shuji Yamashita, Daisuke Ito, Masaharu Tsuchiya

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The distribution of alcohol dehydrogenase in the hepatic acinus was examined by both histochemical and immunohistochemical approaches. The immunohistochemical method using anti–alcohol dehydrogenase antibody indicated zone 3 predominance of this enzyme in the hepatic acinus, whereas a conventional histochemical method showed slight zone 1 predominance. However, when the histochemical technique was improved by using 2% glutaraldehyde instead of formalin for fixation and by adding phenazine methosulfate (0.33 mmol/L) to the staining incubation mixture, this method also supported zone 3 predominance of alcohol dehydrogenase. Evidence for zone 3 distribution of alcohol dehydrogenase may be of value in elucidating the mechanism of zone 3 liver damage by alcohol. (HEPATOLOGY 1990;12:66–69).

Original languageEnglish
Pages (from-to)66-69
Number of pages4
JournalHepatology
Volume12
Issue number1
DOIs
Publication statusPublished - 1990 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Hepatology

Cite this

Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats. / Kato, Shinzo; Ishii, Hiromasa; Aiso, Sadakazu; Yamashita, Shuji; Ito, Daisuke; Tsuchiya, Masaharu.

In: Hepatology, Vol. 12, No. 1, 01.01.1990, p. 66-69.

Research output: Contribution to journalArticle

Kato, Shinzo ; Ishii, Hiromasa ; Aiso, Sadakazu ; Yamashita, Shuji ; Ito, Daisuke ; Tsuchiya, Masaharu. / Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats. In: Hepatology. 1990 ; Vol. 12, No. 1. pp. 66-69.
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