Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats

Shinzo Kato, Hiromasa Ishii, Sadakazu Aiso, Shuji Yamashita, Daisuke Ito, Masaharu Tsuchiya

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The distribution of alcohol dehydrogenase in the hepatic acinus was examined by both histochemical and immunohistochemical approaches. The immunohistochemical method using anti–alcohol dehydrogenase antibody indicated zone 3 predominance of this enzyme in the hepatic acinus, whereas a conventional histochemical method showed slight zone 1 predominance. However, when the histochemical technique was improved by using 2% glutaraldehyde instead of formalin for fixation and by adding phenazine methosulfate (0.33 mmol/L) to the staining incubation mixture, this method also supported zone 3 predominance of alcohol dehydrogenase. Evidence for zone 3 distribution of alcohol dehydrogenase may be of value in elucidating the mechanism of zone 3 liver damage by alcohol. (HEPATOLOGY 1990;12:66–69).

Original languageEnglish
Pages (from-to)66-69
Number of pages4
Issue number1
Publication statusPublished - 1990 Jan 1
Externally publishedYes


ASJC Scopus subject areas

  • Hepatology

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