Identification of UBIAD1 as a novel human menaquinone-4 biosynthetic enzyme

Kimie Nakagawa, Yoshihisa Hirota, Natsumi Sawada, Naohito Yuge, Masato Watanabe, Yuri Uchino, Naoko Okuda, Yuka Shimomura, Yoshitomo Suhara, Toshio Okano

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Vitaming K occurs in the natural world in several forms, including a plant form, phylloquinone (PK), and a bacterial form, menaquinones (MKs). In many species, including humans, PK is a minor constituent of hepatic vitamingK content, with most hepatic vitamingK content comprising long-chain MKs. Menaquinone-4 (MK-4) is ubiquitously present in extrahepatic tissues, with particularly high concentrations in the brain, kidney and pancreas of humans and rats. It has consistently been shown that PK is endogenously converted to MK-4 (refs 4g-8). This occurs either directly within certain tissues or by interconversion to menadione (K 3), followed by prenylation to MK-4 (refs 9g-12). No previous study has sought to identify the human enzyme responsible for MK-4 biosynthesis. Previously we provided evidence for the conversion of PK and K 3 into MK-4 in mouse cerebra. However, the molecular mechanisms for these conversion reactions are unclear. Here we identify a human MK-4 biosynthetic enzyme. We screened the human genome database for prenylation enzymes and found UbiA prenyltransferase containing 1 (UBIAD1), a human homologue of Escherichia coli prenyltransferase menA. We found that short interfering RNA against the UBIAD1 gene inhibited the conversion of deuterium-labelled vitamingK derivatives into deuterium-labelled-MK-4 (MK-4-d 7) in human cells. We confirmed that the UBIAD1 gene encodes an MK-4 biosynthetic enzyme through its expression and conversion of deuterium-labelled vitamingK derivatives into MK-4-d 7 in insect cells infected with UBIAD1 baculovirus. Converted MK-4-d 7 was chemically identified by 2 H-NMR analysis. MK-4 biosynthesis by UBIAD1 was not affected by the vitamingK antagonist warfarin. UBIAD1 was localized in endoplasmic reticulum and ubiquitously expressed in several tissues of mice. Our results show that UBIAD1 is a human MK-4 biosynthetic enzyme; this identification will permit more effective decisions to be made about vitamingK intake and bone health.

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalNature
Volume468
Issue number7320
DOIs
Publication statusPublished - 2010 Nov 4
Externally publishedYes

ASJC Scopus subject areas

  • General

Cite this

Nakagawa, K., Hirota, Y., Sawada, N., Yuge, N., Watanabe, M., Uchino, Y., ... Okano, T. (2010). Identification of UBIAD1 as a novel human menaquinone-4 biosynthetic enzyme. Nature, 468(7320), 117-121. https://doi.org/10.1038/nature09464

Identification of UBIAD1 as a novel human menaquinone-4 biosynthetic enzyme. / Nakagawa, Kimie; Hirota, Yoshihisa; Sawada, Natsumi; Yuge, Naohito; Watanabe, Masato; Uchino, Yuri; Okuda, Naoko; Shimomura, Yuka; Suhara, Yoshitomo; Okano, Toshio.

In: Nature, Vol. 468, No. 7320, 04.11.2010, p. 117-121.

Research output: Contribution to journalArticle

Nakagawa, K, Hirota, Y, Sawada, N, Yuge, N, Watanabe, M, Uchino, Y, Okuda, N, Shimomura, Y, Suhara, Y & Okano, T 2010, 'Identification of UBIAD1 as a novel human menaquinone-4 biosynthetic enzyme', Nature, vol. 468, no. 7320, pp. 117-121. https://doi.org/10.1038/nature09464
Nakagawa K, Hirota Y, Sawada N, Yuge N, Watanabe M, Uchino Y et al. Identification of UBIAD1 as a novel human menaquinone-4 biosynthetic enzyme. Nature. 2010 Nov 4;468(7320):117-121. https://doi.org/10.1038/nature09464
Nakagawa, Kimie ; Hirota, Yoshihisa ; Sawada, Natsumi ; Yuge, Naohito ; Watanabe, Masato ; Uchino, Yuri ; Okuda, Naoko ; Shimomura, Yuka ; Suhara, Yoshitomo ; Okano, Toshio. / Identification of UBIAD1 as a novel human menaquinone-4 biosynthetic enzyme. In: Nature. 2010 ; Vol. 468, No. 7320. pp. 117-121.
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AU - Watanabe, Masato

AU - Uchino, Yuri

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N2 - Vitaming K occurs in the natural world in several forms, including a plant form, phylloquinone (PK), and a bacterial form, menaquinones (MKs). In many species, including humans, PK is a minor constituent of hepatic vitamingK content, with most hepatic vitamingK content comprising long-chain MKs. Menaquinone-4 (MK-4) is ubiquitously present in extrahepatic tissues, with particularly high concentrations in the brain, kidney and pancreas of humans and rats. It has consistently been shown that PK is endogenously converted to MK-4 (refs 4g-8). This occurs either directly within certain tissues or by interconversion to menadione (K 3), followed by prenylation to MK-4 (refs 9g-12). No previous study has sought to identify the human enzyme responsible for MK-4 biosynthesis. Previously we provided evidence for the conversion of PK and K 3 into MK-4 in mouse cerebra. However, the molecular mechanisms for these conversion reactions are unclear. Here we identify a human MK-4 biosynthetic enzyme. We screened the human genome database for prenylation enzymes and found UbiA prenyltransferase containing 1 (UBIAD1), a human homologue of Escherichia coli prenyltransferase menA. We found that short interfering RNA against the UBIAD1 gene inhibited the conversion of deuterium-labelled vitamingK derivatives into deuterium-labelled-MK-4 (MK-4-d 7) in human cells. We confirmed that the UBIAD1 gene encodes an MK-4 biosynthetic enzyme through its expression and conversion of deuterium-labelled vitamingK derivatives into MK-4-d 7 in insect cells infected with UBIAD1 baculovirus. Converted MK-4-d 7 was chemically identified by 2 H-NMR analysis. MK-4 biosynthesis by UBIAD1 was not affected by the vitamingK antagonist warfarin. UBIAD1 was localized in endoplasmic reticulum and ubiquitously expressed in several tissues of mice. Our results show that UBIAD1 is a human MK-4 biosynthetic enzyme; this identification will permit more effective decisions to be made about vitamingK intake and bone health.

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