Immune deficiency due to high copy numbers of an Aβ k transgene

Susan Gilfillan, Sadakazu Aiso, Sara A. Michie, Hugh O. Mcdevitt

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Because allelic polymorphism of the major histocompatibility complex class II antigens affects the immune response at several levels, we wished to characterize the contribution of a particular α or β chain in vivo using transgenic mice. We have established and characterized 12 lines of H-2s/s mice carrying from 1 to 65 copies of an Aβ k transgene. The transgene was coexpressed with the endogenous allele in a tissue-specific manner, and Aβ k mRNA expression correlated well with transgene copy number. High copy number (extreme overexpression) of the transgene was associated with a variety of defects, including a significant reduction in Ia cell-surface expression, a severe decrease in B-cell number, abnormal extramedullary granulopoiesis, and an increased susceptibility to infection. In this paper we describe in detail the phenotype associated with high copy numbers of the Aβ k transgene. The defects we have observed may be relevant to similar phenomena seen in other transgenic mice. In addition, these mice have fortuitously provided a system in which to assess the effect of various levels of class II cell-surface expression in the thymus on selection of the T-cell repertoire.

Original languageEnglish
Pages (from-to)7319-7323
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number18
DOIs
Publication statusPublished - 1990 Jan 1
Externally publishedYes

Keywords

  • Class II
  • Transgenic mice

ASJC Scopus subject areas

  • General

Cite this

Immune deficiency due to high copy numbers of an Aβ k transgene. / Gilfillan, Susan; Aiso, Sadakazu; Michie, Sara A.; Mcdevitt, Hugh O.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 18, 01.01.1990, p. 7319-7323.

Research output: Contribution to journalArticle

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