Immunoglobulin G subclass distribution of human anticolon antibodies in ulcerative colitis

M. OHARA, T. HIBI, N. WATANABE, K. KOBAYASHI, H. TAKAISHI, A. HAYASHI, Y. HOSODA, K. TODA, Y. IWAO, M. WATANABE, Sadakazu Aiso, M. TSUCHIYA, H. ISHII

Research output: Contribution to journalArticle

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Abstract

Abstract Immunoglobulin G (IgG) subclasses of anticolon antibodies were studied in patients with ulcerative colitis (UC) using enzyme‐linked immunosorbent assay (ELISA). The concentrations of total serum IgG subclasses were also measured by ELISA. The values for total serum IgG subclasses in patients with UC were not significantly different from those in normal controls, while the ratio of IgG1 to IgG2 in the patients was significantly higher than that in normal controls. All four IgG subclasses of autoantibodies were demonstrated in the sera of the patients. IgG4 anticolon antibodies were detected most frequently (15 out of 18 patients, 83%). IgG2 was the next most prevalent (9 of 18 patients, 50%). The activity of anticolon antibodies in each subclass did not correlate with the concentration of the corresponding serum IgG subclass. Seven cell lines producing anticolon antibodies were obtained from the colonic mucosa of the patients by Epstein‐Barr virus (EBV) transformation. IgG subclasses of anticolon antibodies secreted by these cell lines were also varied. IgG4 subclass was secreted by three EBV transformed cell lines, all of which produced IgG4 anticolon antibodies. These results suggest that all four different IgG subclasses could respond to the colon antigens and that various antigens in colonic mucosa or lumen may contribute to the induction of those autoantibodies. In addition, the prominence of IgG4 anticolon antibodies may support the pathogenic role of this subclass in UC as in other autoimmune diseases.

Original languageEnglish
Pages (from-to)158-164
Number of pages7
JournalJournal of Gastroenterology and Hepatology
Volume10
Issue number2
DOIs
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Keywords

  • anticolon antibodies
  • Epstein‐Barr virus
  • IgG subclass
  • serum
  • transformed lymphocytes
  • ulcerative colitis.

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

Immunoglobulin G subclass distribution of human anticolon antibodies in ulcerative colitis. / OHARA, M.; HIBI, T.; WATANABE, N.; KOBAYASHI, K.; TAKAISHI, H.; HAYASHI, A.; HOSODA, Y.; TODA, K.; IWAO, Y.; WATANABE, M.; Aiso, Sadakazu; TSUCHIYA, M.; ISHII, H.

In: Journal of Gastroenterology and Hepatology, Vol. 10, No. 2, 01.01.1995, p. 158-164.

Research output: Contribution to journalArticle

OHARA, M, HIBI, T, WATANABE, N, KOBAYASHI, K, TAKAISHI, H, HAYASHI, A, HOSODA, Y, TODA, K, IWAO, Y, WATANABE, M, Aiso, S, TSUCHIYA, M & ISHII, H 1995, 'Immunoglobulin G subclass distribution of human anticolon antibodies in ulcerative colitis', Journal of Gastroenterology and Hepatology, vol. 10, no. 2, pp. 158-164. https://doi.org/10.1111/j.1440-1746.1995.tb01071.x
OHARA, M. ; HIBI, T. ; WATANABE, N. ; KOBAYASHI, K. ; TAKAISHI, H. ; HAYASHI, A. ; HOSODA, Y. ; TODA, K. ; IWAO, Y. ; WATANABE, M. ; Aiso, Sadakazu ; TSUCHIYA, M. ; ISHII, H. / Immunoglobulin G subclass distribution of human anticolon antibodies in ulcerative colitis. In: Journal of Gastroenterology and Hepatology. 1995 ; Vol. 10, No. 2. pp. 158-164.
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abstract = "Abstract Immunoglobulin G (IgG) subclasses of anticolon antibodies were studied in patients with ulcerative colitis (UC) using enzyme‐linked immunosorbent assay (ELISA). The concentrations of total serum IgG subclasses were also measured by ELISA. The values for total serum IgG subclasses in patients with UC were not significantly different from those in normal controls, while the ratio of IgG1 to IgG2 in the patients was significantly higher than that in normal controls. All four IgG subclasses of autoantibodies were demonstrated in the sera of the patients. IgG4 anticolon antibodies were detected most frequently (15 out of 18 patients, 83{\%}). IgG2 was the next most prevalent (9 of 18 patients, 50{\%}). The activity of anticolon antibodies in each subclass did not correlate with the concentration of the corresponding serum IgG subclass. Seven cell lines producing anticolon antibodies were obtained from the colonic mucosa of the patients by Epstein‐Barr virus (EBV) transformation. IgG subclasses of anticolon antibodies secreted by these cell lines were also varied. IgG4 subclass was secreted by three EBV transformed cell lines, all of which produced IgG4 anticolon antibodies. These results suggest that all four different IgG subclasses could respond to the colon antigens and that various antigens in colonic mucosa or lumen may contribute to the induction of those autoantibodies. In addition, the prominence of IgG4 anticolon antibodies may support the pathogenic role of this subclass in UC as in other autoimmune diseases.",
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N2 - Abstract Immunoglobulin G (IgG) subclasses of anticolon antibodies were studied in patients with ulcerative colitis (UC) using enzyme‐linked immunosorbent assay (ELISA). The concentrations of total serum IgG subclasses were also measured by ELISA. The values for total serum IgG subclasses in patients with UC were not significantly different from those in normal controls, while the ratio of IgG1 to IgG2 in the patients was significantly higher than that in normal controls. All four IgG subclasses of autoantibodies were demonstrated in the sera of the patients. IgG4 anticolon antibodies were detected most frequently (15 out of 18 patients, 83%). IgG2 was the next most prevalent (9 of 18 patients, 50%). The activity of anticolon antibodies in each subclass did not correlate with the concentration of the corresponding serum IgG subclass. Seven cell lines producing anticolon antibodies were obtained from the colonic mucosa of the patients by Epstein‐Barr virus (EBV) transformation. IgG subclasses of anticolon antibodies secreted by these cell lines were also varied. IgG4 subclass was secreted by three EBV transformed cell lines, all of which produced IgG4 anticolon antibodies. These results suggest that all four different IgG subclasses could respond to the colon antigens and that various antigens in colonic mucosa or lumen may contribute to the induction of those autoantibodies. In addition, the prominence of IgG4 anticolon antibodies may support the pathogenic role of this subclass in UC as in other autoimmune diseases.

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