Immunohistochemical Study of Gamma-Glutamyl Transpeptidase with Monoclonal Antibodies. II. An Immunoelectron Microscopic Study in Rat Kidney

Shuji Yamashita; Sadakazu Aiso; Masahide Shiozawa; Kenjiro Yasuda

doi:10.1267/ahc.22.367

Immunohistochemical Study of Gamma-Glutamyl Transpeptidase with Monoclonal Antibodies. II. An Immunoelectron Microscopic Study in Rat Kidney

Shuji Yamashita, Sadakazu Aiso, Masahide Shiozawa, Kenjiro Yasuda

SIT Research Laboratories

Research output: Contribution to journal › Article

6 Citations (Scopus)

Abstract

The localization of gamma-glutamyl transpeptidase (γ-GTP) in the rat kidney was examined by immunoelectron microscopy using monoclonal antibody to rat γ- GTP. The enzyme was distributed along the brush border and in the apical canaliculi and vacuoles of the proximal tubules. The immunoreaction of γ-GTP in the brush borders increased from the S₁ to S₃ segments in the proximal tubules. Conspicuousγ;-GTP immunostaining was observed along the basolateral membranes of the S₂ and S₃ segments of the proximal tubules. In addition, the apical cell membrane of the upper part of descending thin limbs of Henle's loops exhibited positive γ-GTP immunostaining. A faint reaction was occasionally recognized in the apical cell membranes of the distal convoluted cells, including the macula densa.

Original language	English
Pages (from-to)	367-374
Number of pages	8
Journal	Acta Histochemica et Cytochemica
Volume	22
Issue number	3
DOIs	https://doi.org/10.1267/ahc.22.367
Publication status	Published - 1989 Jan 1

ASJC Scopus subject areas

Pathology and Forensic Medicine
Biochemistry
Physiology
Histology
Cell Biology

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Yamashita, S., Aiso, S., Shiozawa, M., & Yasuda, K. (1989). Immunohistochemical Study of Gamma-Glutamyl Transpeptidase with Monoclonal Antibodies. II. An Immunoelectron Microscopic Study in Rat Kidney. Acta Histochemica et Cytochemica, 22(3), 367-374. https://doi.org/10.1267/ahc.22.367

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abstract = "The localization of gamma-glutamyl transpeptidase (γ-GTP) in the rat kidney was examined by immunoelectron microscopy using monoclonal antibody to rat γ- GTP. The enzyme was distributed along the brush border and in the apical canaliculi and vacuoles of the proximal tubules. The immunoreaction of γ-GTP in the brush borders increased from the S1 to S3 segments in the proximal tubules. Conspicuousγ;-GTP immunostaining was observed along the basolateral membranes of the S2 and S3 segments of the proximal tubules. In addition, the apical cell membrane of the upper part of descending thin limbs of Henle's loops exhibited positive γ-GTP immunostaining. A faint reaction was occasionally recognized in the apical cell membranes of the distal convoluted cells, including the macula densa.",

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