Abstract
The localization of gamma-glutamyl transpeptidase (γ-GTP) in the rat kidney was examined by immunoelectron microscopy using monoclonal antibody to rat γ- GTP. The enzyme was distributed along the brush border and in the apical canaliculi and vacuoles of the proximal tubules. The immunoreaction of γ-GTP in the brush borders increased from the S1 to S3 segments in the proximal tubules. Conspicuousγ;-GTP immunostaining was observed along the basolateral membranes of the S2 and S3 segments of the proximal tubules. In addition, the apical cell membrane of the upper part of descending thin limbs of Henle's loops exhibited positive γ-GTP immunostaining. A faint reaction was occasionally recognized in the apical cell membranes of the distal convoluted cells, including the macula densa.
| Original language | English |
|---|---|
| Pages (from-to) | 367-374 |
| Number of pages | 8 |
| Journal | Acta Histochemica et Cytochemica |
| Volume | 22 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1989 Jan 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Biochemistry
- Physiology
- Histology
- Cell Biology
Cite this
Immunohistochemical Study of Gamma-Glutamyl Transpeptidase with Monoclonal Antibodies. II. An Immunoelectron Microscopic Study in Rat Kidney. / Yamashita, Shuji; Aiso, Sadakazu; Shiozawa, Masahide; Yasuda, Kenjiro.
In: Acta Histochemica et Cytochemica, Vol. 22, No. 3, 01.01.1989, p. 367-374.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Immunohistochemical Study of Gamma-Glutamyl Transpeptidase with Monoclonal Antibodies. II. An Immunoelectron Microscopic Study in Rat Kidney
AU - Yamashita, Shuji
AU - Aiso, Sadakazu
AU - Shiozawa, Masahide
AU - Yasuda, Kenjiro
PY - 1989/1/1
Y1 - 1989/1/1
N2 - The localization of gamma-glutamyl transpeptidase (γ-GTP) in the rat kidney was examined by immunoelectron microscopy using monoclonal antibody to rat γ- GTP. The enzyme was distributed along the brush border and in the apical canaliculi and vacuoles of the proximal tubules. The immunoreaction of γ-GTP in the brush borders increased from the S1 to S3 segments in the proximal tubules. Conspicuousγ;-GTP immunostaining was observed along the basolateral membranes of the S2 and S3 segments of the proximal tubules. In addition, the apical cell membrane of the upper part of descending thin limbs of Henle's loops exhibited positive γ-GTP immunostaining. A faint reaction was occasionally recognized in the apical cell membranes of the distal convoluted cells, including the macula densa.
AB - The localization of gamma-glutamyl transpeptidase (γ-GTP) in the rat kidney was examined by immunoelectron microscopy using monoclonal antibody to rat γ- GTP. The enzyme was distributed along the brush border and in the apical canaliculi and vacuoles of the proximal tubules. The immunoreaction of γ-GTP in the brush borders increased from the S1 to S3 segments in the proximal tubules. Conspicuousγ;-GTP immunostaining was observed along the basolateral membranes of the S2 and S3 segments of the proximal tubules. In addition, the apical cell membrane of the upper part of descending thin limbs of Henle's loops exhibited positive γ-GTP immunostaining. A faint reaction was occasionally recognized in the apical cell membranes of the distal convoluted cells, including the macula densa.
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UR - http://www.scopus.com/inward/citedby.url?scp=12344329629&partnerID=8YFLogxK
U2 - 10.1267/ahc.22.367
DO - 10.1267/ahc.22.367
M3 - Article
AN - SCOPUS:12344329629
VL - 22
SP - 367
EP - 374
JO - Acta Histochemica et Cytochemica
JF - Acta Histochemica et Cytochemica
SN - 0044-5991
IS - 3
ER -