Immunohistochemical Study of Gamma-Glutamyl Transpeptidase with Monoclonal Antibodies. II. An Immunoelectron Microscopic Study in Rat Kidney

Shuji Yamashita; Sadakazu Aiso; Masahide Shiozawa; Kenjiro Yasuda

doi:10.1267/ahc.22.367

Immunohistochemical Study of Gamma-Glutamyl Transpeptidase with Monoclonal Antibodies. II. An Immunoelectron Microscopic Study in Rat Kidney

Shuji Yamashita, Sadakazu Aiso, Masahide Shiozawa, Kenjiro Yasuda

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6 Citations (Scopus)

Abstract

The localization of gamma-glutamyl transpeptidase (γ-GTP) in the rat kidney was examined by immunoelectron microscopy using monoclonal antibody to rat γ- GTP. The enzyme was distributed along the brush border and in the apical canaliculi and vacuoles of the proximal tubules. The immunoreaction of γ-GTP in the brush borders increased from the S₁ to S₃ segments in the proximal tubules. Conspicuousγ;-GTP immunostaining was observed along the basolateral membranes of the S₂ and S₃ segments of the proximal tubules. In addition, the apical cell membrane of the upper part of descending thin limbs of Henle's loops exhibited positive γ-GTP immunostaining. A faint reaction was occasionally recognized in the apical cell membranes of the distal convoluted cells, including the macula densa.

Original language	English
Pages (from-to)	367-374
Number of pages	8
Journal	Acta Histochemica et Cytochemica
Volume	22
Issue number	3
DOIs	https://doi.org/10.1267/ahc.22.367
Publication status	Published - 1989 Jan 1
Externally published	Yes

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ASJC Scopus subject areas

Pathology and Forensic Medicine
Biochemistry
Physiology
Histology
Cell Biology

Cite this

Yamashita, S., Aiso, S., Shiozawa, M., & Yasuda, K. (1989). Immunohistochemical Study of Gamma-Glutamyl Transpeptidase with Monoclonal Antibodies. II. An Immunoelectron Microscopic Study in Rat Kidney. Acta Histochemica et Cytochemica, 22(3), 367-374. https://doi.org/10.1267/ahc.22.367

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10.1267/ahc.22.367