Immunohistochemical study of localization of γ-glutamyl transpeptidase in the rat brain

M. Ogawa; M. Shiozawa; Y. Hiraoka; Y. Takeuchi; S. Aiso

doi:10.1016/S0040-8166(98)80078-5

Immunohistochemical study of localization of γ-glutamyl transpeptidase in the rat brain

M. Ogawa, M. Shiozawa, Y. Hiraoka, Y. Takeuchi, S. Aiso

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

γ-glutamyl transpeptidase (γ-GTP) is a membrane-bound enzyme which is known to play a crucial role in active transport of amino acids across membrane barriers. We prepared a monoclonal antibody recognizing specifically rat γ-GTP and investigated localization of the enzyme in the rat brain by immunohistochemistry with this antibody. The antigen was localized on the ependyma, epithelia of the choroid plexus and microvessels. More precise localization of γ-GTP was examined with immuno-electron microscopy. The antigen was recognized on the microvilli and cilia of the ependymal cells, microvilli of the choroid epithelial cells and luminal membranes of the vascular endothelial cells.

Original language	English
Pages (from-to)	597-601
Number of pages	5
Journal	Tissue and Cell
Volume	30
Issue number	6
DOIs	https://doi.org/10.1016/S0040-8166(98)80078-5
Publication status	Published - 1998 Jan 1
Externally published	Yes

Keywords

Choroid plexus
Ependymal cell
Immunohistochemistry
Vascular endothelial cell
γ-glutamyl transpeptidase

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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title = "Immunohistochemical study of localization of γ-glutamyl transpeptidase in the rat brain",

abstract = "γ-glutamyl transpeptidase (γ-GTP) is a membrane-bound enzyme which is known to play a crucial role in active transport of amino acids across membrane barriers. We prepared a monoclonal antibody recognizing specifically rat γ-GTP and investigated localization of the enzyme in the rat brain by immunohistochemistry with this antibody. The antigen was localized on the ependyma, epithelia of the choroid plexus and microvessels. More precise localization of γ-GTP was examined with immuno-electron microscopy. The antigen was recognized on the microvilli and cilia of the ependymal cells, microvilli of the choroid epithelial cells and luminal membranes of the vascular endothelial cells.",

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AU - Ogawa, M.

AU - Shiozawa, M.

AU - Hiraoka, Y.

AU - Takeuchi, Y.

AU - Aiso, S.

PY - 1998/1/1

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KW - Ependymal cell

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