Immunohistochemical study of localization of γ-glutamyl transpeptidase in the rat brain

M. Ogawa; M. Shiozawa; Y. Hiraoka; Y. Takeuchi; S. Aiso

doi:10.1016/S0040-8166(98)80078-5

Immunohistochemical study of localization of γ-glutamyl transpeptidase in the rat brain

M. Ogawa, M. Shiozawa, Y. Hiraoka, Y. Takeuchi, S. Aiso

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

γ-glutamyl transpeptidase (γ-GTP) is a membrane-bound enzyme which is known to play a crucial role in active transport of amino acids across membrane barriers. We prepared a monoclonal antibody recognizing specifically rat γ-GTP and investigated localization of the enzyme in the rat brain by immunohistochemistry with this antibody. The antigen was localized on the ependyma, epithelia of the choroid plexus and microvessels. More precise localization of γ-GTP was examined with immuno-electron microscopy. The antigen was recognized on the microvilli and cilia of the ependymal cells, microvilli of the choroid epithelial cells and luminal membranes of the vascular endothelial cells.

Original language	English
Pages (from-to)	597-601
Number of pages	5
Journal	Tissue and Cell
Volume	30
Issue number	6
DOIs	https://doi.org/10.1016/S0040-8166(98)80078-5
Publication status	Published - 1998
Externally published	Yes

Keywords

Choroid plexus
Ependymal cell
Immunohistochemistry
Vascular endothelial cell
γ-glutamyl transpeptidase

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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title = "Immunohistochemical study of localization of γ-glutamyl transpeptidase in the rat brain",

abstract = "γ-glutamyl transpeptidase (γ-GTP) is a membrane-bound enzyme which is known to play a crucial role in active transport of amino acids across membrane barriers. We prepared a monoclonal antibody recognizing specifically rat γ-GTP and investigated localization of the enzyme in the rat brain by immunohistochemistry with this antibody. The antigen was localized on the ependyma, epithelia of the choroid plexus and microvessels. More precise localization of γ-GTP was examined with immuno-electron microscopy. The antigen was recognized on the microvilli and cilia of the ependymal cells, microvilli of the choroid epithelial cells and luminal membranes of the vascular endothelial cells.",

keywords = "Choroid plexus, Ependymal cell, Immunohistochemistry, Vascular endothelial cell, γ-glutamyl transpeptidase",

author = "M. Ogawa and M. Shiozawa and Y. Hiraoka and Y. Takeuchi and S. Aiso",

note = "Funding Information: This work was supported, in part, by a grant from Ministry of Education, Science and Culture to S. A., and by Keio Gakuji Fukuzawa Memorial Fund for the Advancement of Education and Research from Keio University to Y. H. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "1998",

doi = "10.1016/S0040-8166(98)80078-5",

language = "English",

volume = "30",

pages = "597--601",

journal = "Tissue and Cell",

issn = "0040-8166",

publisher = "Churchill Livingstone",

number = "6",

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T1 - Immunohistochemical study of localization of γ-glutamyl transpeptidase in the rat brain

AU - Ogawa, M.

AU - Shiozawa, M.

AU - Hiraoka, Y.

AU - Takeuchi, Y.

AU - Aiso, S.

N1 - Funding Information: This work was supported, in part, by a grant from Ministry of Education, Science and Culture to S. A., and by Keio Gakuji Fukuzawa Memorial Fund for the Advancement of Education and Research from Keio University to Y. H. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 1998

Y1 - 1998

N2 - γ-glutamyl transpeptidase (γ-GTP) is a membrane-bound enzyme which is known to play a crucial role in active transport of amino acids across membrane barriers. We prepared a monoclonal antibody recognizing specifically rat γ-GTP and investigated localization of the enzyme in the rat brain by immunohistochemistry with this antibody. The antigen was localized on the ependyma, epithelia of the choroid plexus and microvessels. More precise localization of γ-GTP was examined with immuno-electron microscopy. The antigen was recognized on the microvilli and cilia of the ependymal cells, microvilli of the choroid epithelial cells and luminal membranes of the vascular endothelial cells.

AB - γ-glutamyl transpeptidase (γ-GTP) is a membrane-bound enzyme which is known to play a crucial role in active transport of amino acids across membrane barriers. We prepared a monoclonal antibody recognizing specifically rat γ-GTP and investigated localization of the enzyme in the rat brain by immunohistochemistry with this antibody. The antigen was localized on the ependyma, epithelia of the choroid plexus and microvessels. More precise localization of γ-GTP was examined with immuno-electron microscopy. The antigen was recognized on the microvilli and cilia of the ependymal cells, microvilli of the choroid epithelial cells and luminal membranes of the vascular endothelial cells.

KW - Choroid plexus

KW - Ependymal cell

KW - Immunohistochemistry

KW - Vascular endothelial cell

KW - γ-glutamyl transpeptidase

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JO - Tissue and Cell

JF - Tissue and Cell

SN - 0040-8166

IS - 6

ER -

Immunohistochemical study of localization of γ-glutamyl transpeptidase in the rat brain

Abstract

Keywords

ASJC Scopus subject areas

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