Importance of 3′-hydroxyl group of the nucleosides for the reactivity of thymidine phosphorylase from Escherichia coli

Akihiko Hatano, Aiko Harano, Masayuki Kirihara

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Abstract

Thymidine phosphorylase in phosphate buffer catalyzed the conversion of thymidine to unnatural nucleosides. The 3′-OH, but not the 5′-OH of ribosyl moiety is necessary to be recognized as a substrate. Thus 3′-deoxythymidine could not convert to 5-fluorouracil-2′,3′- dideoxyribose. However, 5′-deoxythymidine was converted to 5-fluorouracil-2′,5′-dideoxyribose.

Original languageEnglish
Pages (from-to)232-233
Number of pages2
JournalChemistry Letters
Volume35
Issue number2
DOIs
Publication statusPublished - 2006 Feb 5

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ASJC Scopus subject areas

  • Chemistry(all)

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