In vitro cytotoxicity of carbon black nanoparticles synthesized from solution plasma on human lung fibroblast cells

Gasidit Panomsuwan, Chayanaphat Chokradjaroen, Ratana Rujiravanit, Tomonaga Ueno, Nagahiro Saito

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

Carbon black nanoparticles (CB-NPs) have been synthesized from liquid benzene by a solution plasma method at room temperature and atmospheric pressure. The morphological observation by scanning electron microscopy revealed the agglomeration of aggregated fine particles. The synthesized CB-NPs were predominantly amorphous as confirmed by X-ray diffraction. The in vitro cytotoxicity of CB-NPs on the human lung fibroblast (MRC-5) cell line was assessed by the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and systematically compared with those of two types of commercial carbon blacks (i.e., Vulcan XC-72 and Ketjenblack EC-600JD). Cell viabilities were studied at different concentrations of 32.5, 65, 125, and 250 μg/mL. It was found that the CB-NPs derived from solution plasma exhibited a lower cytotoxicity on the MRC-5 cells than the other two comparative carbon blacks. The viability of MRC-5 cells exposed to CB-NPs remained higher than 90% even at a high concentration of 250 μg/mL. This result preliminarily confirmed the biosafety and potential use of CB-NPs in the field of biological applications.

Original languageEnglish
Article number0102BG
JournalJapanese Journal of Applied Physics
Volume57
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1
Externally publishedYes

Fingerprint

fibroblasts
Fibroblasts
Cytotoxicity
Carbon black
lungs
Cells
Nanoparticles
Plasmas
nanoparticles
carbon
viability
agglomeration
cells
cultured cells
Atmospheric pressure
bromides
Assays
Benzene
atmospheric pressure
Agglomeration

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

In vitro cytotoxicity of carbon black nanoparticles synthesized from solution plasma on human lung fibroblast cells. / Panomsuwan, Gasidit; Chokradjaroen, Chayanaphat; Rujiravanit, Ratana; Ueno, Tomonaga; Saito, Nagahiro.

In: Japanese Journal of Applied Physics, Vol. 57, No. 1, 0102BG, 01.01.2018.

Research output: Contribution to journalReview article

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