In vivo layer visualization of rat olfactory bulb by a swept source optical coherence tomography and its confirmation through electrocoagulation and anatomy

Hideyuki Watanabe, Rajagopalan Umamaheswari, Yu Nakamichi, Kei M. Igarashi, Violeta Dimitrova Madjarova, Hirofumi Kadono, Manabu Tanifuji

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Here, we report in vivo 3-D visualization of the layered organization of a rat olfactory bulb (OB) by a swept source optical coherence tomography (SS-OCT). The SS-OCT operates at a wavelength of 1334 nm with respective theoretical depth and lateral resolutions of 6.7 μm and 15.4 μm in air and hence it is possible to get a 3D structural map of OB in vivo at the micron level resolution with millimeter-scale imaging depth. Up until now, with methods such as MRI, confocal microscopy, OB depth structure in vivo had not been clearly visualized as these do not satisfy the criterion of simultaneously providing micron-scale spatial resolution and imaging up to a few millimeter in depth. In order to confirm the OB's layered organization revealed by SS-OCT, we introduced the technique of electrocoagulation to make landmarks across the layered structure. To our knowledge this is such a first study that combines electrocoagulation and OCT in vivo of rat OB. Our results confirmed the layered organization of OB, and moreover the layers were clearly identified by electrocoagulation landmarks both in the OCT structural and anatomical slice images. We expect such a combined study is beneficial for both OCT and neuroscience fields.

Original languageEnglish
Pages (from-to)2279-2287
Number of pages9
JournalBiomedical Optics Express
Volume2
Issue number8
DOIs
Publication statusPublished - 2011 Aug 1
Externally publishedYes

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bulbs
anatomy
rats
tomography
landmarks
neurology
spatial resolution
microscopy
air
wavelengths

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

In vivo layer visualization of rat olfactory bulb by a swept source optical coherence tomography and its confirmation through electrocoagulation and anatomy. / Watanabe, Hideyuki; Umamaheswari, Rajagopalan; Nakamichi, Yu; Igarashi, Kei M.; Madjarova, Violeta Dimitrova; Kadono, Hirofumi; Tanifuji, Manabu.

In: Biomedical Optics Express, Vol. 2, No. 8, 01.08.2011, p. 2279-2287.

Research output: Contribution to journalArticle

Watanabe, Hideyuki ; Umamaheswari, Rajagopalan ; Nakamichi, Yu ; Igarashi, Kei M. ; Madjarova, Violeta Dimitrova ; Kadono, Hirofumi ; Tanifuji, Manabu. / In vivo layer visualization of rat olfactory bulb by a swept source optical coherence tomography and its confirmation through electrocoagulation and anatomy. In: Biomedical Optics Express. 2011 ; Vol. 2, No. 8. pp. 2279-2287.
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