In vivo measurement of morphometric and hemodynamic changes in the microcirculation during angiogenesis under chronic α1-adrenergic blocker treatment

Shigeru Ichioka, Masahiro Shibata, Keisuke Kosaki, Yuko Sato, Kiyonori Harii, Akira Kamiya

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20 Citations (Scopus)

Abstract

The effect of chronic administration of the α 1 blocker prazosin on microvascular angiogenesis was studied in rabbit ear chamber to investigate the role of concomitant increase in flow-oriented wall shear stress in vascular remodeling during angiogenesis. Rabbits were treated with prazosin hydrochloride (50 mg/liter in water) orally after ear chambers were installed. The microcirculation in the chamber was observed and recorded from 4 to 23 postoperative day (POD) by an intravital videomicroscope. The total vascular area (TA) were quantified as a morphometric parameter. Changes in wall shear stress in venules (20-40 μm ID) were calculated from flow velocity, vessel diameter, and in vivo blood viscosity. TA was significantly increased in the prazosin-treated animals. The levels of shear stress, which was increased 1.43 times by prazosin on 9 POD, gradually decreased toward the control level on 13 POD and they almost coincided on 21 POD. These findings experimentally provided supporting evidence that shear stress is probably involved in the adaptive response as one of mechanical factors regulating vascular remodeling during angiogenesis.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalMicrovascular Research
Volume55
Issue number2
DOIs
Publication statusPublished - 1998 Mar
Externally publishedYes

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Keywords

  • Blood flow
  • Intravital microscope
  • Rabbit ear chamber
  • Remodeling
  • Shear stress

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

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