In vivo microvascular oxygen measurements by phosphorescence quenching technique

M. Shibata, S. Ichioka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To clarify the oxygen transport across the microvessels and oxygen distribution in the skeletal muscle, we have newly designed an intravital laser microscopy with oxygen-dependent quenching of phosphorescence technique to determine both the microvascular and the tissue pO2. After injecting the phosphorescent probe into systemic blood, phosphorescence excited by a N2/dye pulse laser was detected with a photomultiplier over a 10 μm in diameter area. In vivo oxygen tension (pO2) measurements revealed the efficiency of this method. This method was then used to measure the pO2 of microvessels with different diameters (40-130 μm) and of interstitial spaces in rat cremaster muscle. These measurements showed a significant reduction of pO2 levels in arterioles of different orders and the presence of large pO2 gradients at the blood/tissue interface of arterioles. These findings suggest that oxygen can be released from larger arterioles and O2 consumption of rat cremaster muscle might be high.

Original languageEnglish
Title of host publication2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
DOIs
Publication statusPublished - 2006 Dec 1
Event2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS - Nagoya, Japan
Duration: 2006 Nov 52006 Nov 8

Publication series

Name2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS

Conference

Conference2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
CountryJapan
CityNagoya
Period06/11/506/11/8

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ASJC Scopus subject areas

  • Computational Mechanics
  • Control and Systems Engineering
  • Human Factors and Ergonomics

Cite this

Shibata, M., & Ichioka, S. (2006). In vivo microvascular oxygen measurements by phosphorescence quenching technique. In 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS [4110392] (2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS). https://doi.org/10.1109/MHS.2006.320286