In vivo model for visualizing flap microcirculation of ischemia-reperfusion

Shigeru Ichioka, Trinh Cao Minh, Masahiro Shibata, Takashi Nakatsuka, Naomi Sekiya, Joji Ando, Kiyonori Harii

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Since ischemia-reperfusion injury continues to be a major problem in reconstructive microsurgery, improvement of experimental models is still desirable. We developed a model that allows direct visualization of flap microcirculation in mice by intravital microscopic techniques. A newly designed skinfold chamber was installed on the dorsum of mice, and microcirculation was inspected with an intravital microscope. An island flap, nourished by the deep circumflex iliac arteries, was elevated after implantation of the chamber, allowing visualization of the microcirculation in the island flap. The island flap was exposed to global ischemia by clamping the pedicles, and the clamps were then released to allow reperfusion. Various microcirculatory responses induced by ischemia-reperfusion were visualized. This model accurately simulated the clinical situation in reconstructive surgery and successfully realized chronic visualization of the flap micro-circulation in vivo.

Original languageEnglish
Pages (from-to)304-310
Number of pages7
JournalMicrosurgery
Volume22
Issue number7
DOIs
Publication statusPublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

Cite this

Ichioka, S., Minh, T. C., Shibata, M., Nakatsuka, T., Sekiya, N., Ando, J., & Harii, K. (2002). In vivo model for visualizing flap microcirculation of ischemia-reperfusion. Microsurgery, 22(7), 304-310. https://doi.org/10.1002/micr.10050

In vivo model for visualizing flap microcirculation of ischemia-reperfusion. / Ichioka, Shigeru; Minh, Trinh Cao; Shibata, Masahiro; Nakatsuka, Takashi; Sekiya, Naomi; Ando, Joji; Harii, Kiyonori.

In: Microsurgery, Vol. 22, No. 7, 2002, p. 304-310.

Research output: Contribution to journalArticle

Ichioka, S, Minh, TC, Shibata, M, Nakatsuka, T, Sekiya, N, Ando, J & Harii, K 2002, 'In vivo model for visualizing flap microcirculation of ischemia-reperfusion', Microsurgery, vol. 22, no. 7, pp. 304-310. https://doi.org/10.1002/micr.10050
Ichioka, Shigeru ; Minh, Trinh Cao ; Shibata, Masahiro ; Nakatsuka, Takashi ; Sekiya, Naomi ; Ando, Joji ; Harii, Kiyonori. / In vivo model for visualizing flap microcirculation of ischemia-reperfusion. In: Microsurgery. 2002 ; Vol. 22, No. 7. pp. 304-310.
@article{bb511c6dd5da436c952d2d41c8ead92b,
title = "In vivo model for visualizing flap microcirculation of ischemia-reperfusion",
abstract = "Since ischemia-reperfusion injury continues to be a major problem in reconstructive microsurgery, improvement of experimental models is still desirable. We developed a model that allows direct visualization of flap microcirculation in mice by intravital microscopic techniques. A newly designed skinfold chamber was installed on the dorsum of mice, and microcirculation was inspected with an intravital microscope. An island flap, nourished by the deep circumflex iliac arteries, was elevated after implantation of the chamber, allowing visualization of the microcirculation in the island flap. The island flap was exposed to global ischemia by clamping the pedicles, and the clamps were then released to allow reperfusion. Various microcirculatory responses induced by ischemia-reperfusion were visualized. This model accurately simulated the clinical situation in reconstructive surgery and successfully realized chronic visualization of the flap micro-circulation in vivo.",
author = "Shigeru Ichioka and Minh, {Trinh Cao} and Masahiro Shibata and Takashi Nakatsuka and Naomi Sekiya and Joji Ando and Kiyonori Harii",
year = "2002",
doi = "10.1002/micr.10050",
language = "English",
volume = "22",
pages = "304--310",
journal = "Microsurgery",
issn = "0738-1085",
publisher = "Wiley-Liss Inc.",
number = "7",

}

TY - JOUR

T1 - In vivo model for visualizing flap microcirculation of ischemia-reperfusion

AU - Ichioka, Shigeru

AU - Minh, Trinh Cao

AU - Shibata, Masahiro

AU - Nakatsuka, Takashi

AU - Sekiya, Naomi

AU - Ando, Joji

AU - Harii, Kiyonori

PY - 2002

Y1 - 2002

N2 - Since ischemia-reperfusion injury continues to be a major problem in reconstructive microsurgery, improvement of experimental models is still desirable. We developed a model that allows direct visualization of flap microcirculation in mice by intravital microscopic techniques. A newly designed skinfold chamber was installed on the dorsum of mice, and microcirculation was inspected with an intravital microscope. An island flap, nourished by the deep circumflex iliac arteries, was elevated after implantation of the chamber, allowing visualization of the microcirculation in the island flap. The island flap was exposed to global ischemia by clamping the pedicles, and the clamps were then released to allow reperfusion. Various microcirculatory responses induced by ischemia-reperfusion were visualized. This model accurately simulated the clinical situation in reconstructive surgery and successfully realized chronic visualization of the flap micro-circulation in vivo.

AB - Since ischemia-reperfusion injury continues to be a major problem in reconstructive microsurgery, improvement of experimental models is still desirable. We developed a model that allows direct visualization of flap microcirculation in mice by intravital microscopic techniques. A newly designed skinfold chamber was installed on the dorsum of mice, and microcirculation was inspected with an intravital microscope. An island flap, nourished by the deep circumflex iliac arteries, was elevated after implantation of the chamber, allowing visualization of the microcirculation in the island flap. The island flap was exposed to global ischemia by clamping the pedicles, and the clamps were then released to allow reperfusion. Various microcirculatory responses induced by ischemia-reperfusion were visualized. This model accurately simulated the clinical situation in reconstructive surgery and successfully realized chronic visualization of the flap micro-circulation in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0036032081&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036032081&partnerID=8YFLogxK

U2 - 10.1002/micr.10050

DO - 10.1002/micr.10050

M3 - Article

VL - 22

SP - 304

EP - 310

JO - Microsurgery

JF - Microsurgery

SN - 0738-1085

IS - 7

ER -