Increased F2-isoprostane levels in the rat brain and plasma caused by oxidative stress and aging, and inhibitory effect of vitamin E

Tomoko Nishio, Ryota Miyadera, Ryuta Sakai, Kouichi Abe, Hideko Kanazawa, Koji Fukui, Shiro Urano

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To determine whether lipid peroxidation induced by reactive oxygen species (ROS) is a causal factor of neurodegeneration during brain aging, we investigated whether F2-isoprostanes, non-cyclooxygenase-derived prostanoids, are formed in the rat brain and plasma by hyperoxia as oxidative stress, and whether their formation is associated with vitamin E status in vivo in association with changes that occur during brain aging. Young rats subjected to hyperoxia for 48 h revealed a marked increase in the levels of F2-isoprostanes in the brain, but not in plasma. A similar increase in F2-isoprostane level was observed in aged rats kept in normal atmosphere. Vitamin E supplementation to young rats markedly inhibited F2-isoprostane formation even after hyperoxia. In contrast, vitamin E-deficient young rats kept in normal atmosphere showed a significant increase in F2-isoprostane level in the brain. These findings indicate that F2-isoprostane formation in the brain has important implications in the etiology of neurodegenerative diseases including Alzheimer's disease during aging, and that the analysis of F2-isoprostane level in plasma does not always reflect neuronal damage cause by oxidative stress. Vitamin E may protect neuronal damage in the brain caused by oxidative stress experienced for a long period during aging.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalJournal of Clinical Biochemistry and Nutrition
Issue number3
Publication statusPublished - 2006 May 1



  • Brain
  • F2-isoprostane
  • Neurodegeneration
  • Oxidative stress
  • Vitamin E

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry

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