Influence of oxidative stress on fusion of pre-synaptic plasma membranes of the rat brain with phosphatidyl choline liposomes, and protective effect of vitamin E

Nao Omi Omoi, Masaya Arai, Mitsunari Saito, Hirokatsu Takatsu, Akira Shibata, Kenji Fukuzawa, Kazue Sato, Kouichi Abe, Koji Fukui, Shiro Urano

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Influence of oxidative stress on fusion of pre-synaptic plasma membranes with phosphatidylcholine (PC) liposomes as a model of synaptic vesicle was investigated. The inhibitory effect of vitamin E on the decline in the fusion caused by oxidative stress was also assessed. Rats subjected to hyperoxia as oxidative stress showed significant increases in the levels of lipid hydroperoxides and protein carbonyl moieties in pre-synaptic plasma membranes in the brain. The ζ potential of pre-synaptic membrane surface was decreased markedly. When synaptosomes were incubated with PC liposomes labeled by either rhodamine B or calcein as a fluorescence probe, or 12-doxyl stearic acid as an ESR spin trapping agent, translocation of each probe into oxidatively damaged pre-synaptic membranes was decreased significantly. Fatty acid composition analysis in pre-synaptic membranes obtained from normal rats revealed a marked increase in linoleic acid and a moderate decrease in docosahexaenoic content after the incubation with liposomes. However, rats subjected to hyperoxia did not show marked changes in these fatty acid contents in their pre-synaptic membranes after the incubation. Such changes caused by hyperoxia were inhibited by vitamin E treatment of rats. These results suggest that oxidative damage of pre-synaptic membranes caused by oxidative stress lowers the lipid-mixing for the membrane fusion. The results of this study imply that vitamin E prevents the deficit in neurotransmission at nerve terminals due to the decline in fusion between pre-synaptic membrane and synaptic vesicles caused by oxidative membrane damage.

Original languageEnglish
Pages (from-to)248-255
Number of pages8
JournalJournal of Nutritional Science and Vitaminology
Volume52
Issue number4
Publication statusPublished - 2006 Aug

Fingerprint

lipid bodies
choline
hyperoxia
protective effect
vitamin E
oxidative stress
plasma membrane
brain
rats
phosphatidylcholines
fatty acid composition
synaptosomes
hydroperoxides
lipids
stearic acid
linoleic acid
nerve tissue
fluorescence
proteins
synaptic vesicles

Keywords

  • Lipid-mixing
  • Neurotransmission
  • Oxidative damage
  • Synaptic membrane fusion
  • Vitamin E

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Influence of oxidative stress on fusion of pre-synaptic plasma membranes of the rat brain with phosphatidyl choline liposomes, and protective effect of vitamin E. / Omoi, Nao Omi; Arai, Masaya; Saito, Mitsunari; Takatsu, Hirokatsu; Shibata, Akira; Fukuzawa, Kenji; Sato, Kazue; Abe, Kouichi; Fukui, Koji; Urano, Shiro.

In: Journal of Nutritional Science and Vitaminology, Vol. 52, No. 4, 08.2006, p. 248-255.

Research output: Contribution to journalArticle

Omoi, Nao Omi ; Arai, Masaya ; Saito, Mitsunari ; Takatsu, Hirokatsu ; Shibata, Akira ; Fukuzawa, Kenji ; Sato, Kazue ; Abe, Kouichi ; Fukui, Koji ; Urano, Shiro. / Influence of oxidative stress on fusion of pre-synaptic plasma membranes of the rat brain with phosphatidyl choline liposomes, and protective effect of vitamin E. In: Journal of Nutritional Science and Vitaminology. 2006 ; Vol. 52, No. 4. pp. 248-255.
@article{98a9ba8c28ad435cbac7a1e5b224b749,
title = "Influence of oxidative stress on fusion of pre-synaptic plasma membranes of the rat brain with phosphatidyl choline liposomes, and protective effect of vitamin E",
abstract = "Influence of oxidative stress on fusion of pre-synaptic plasma membranes with phosphatidylcholine (PC) liposomes as a model of synaptic vesicle was investigated. The inhibitory effect of vitamin E on the decline in the fusion caused by oxidative stress was also assessed. Rats subjected to hyperoxia as oxidative stress showed significant increases in the levels of lipid hydroperoxides and protein carbonyl moieties in pre-synaptic plasma membranes in the brain. The ζ potential of pre-synaptic membrane surface was decreased markedly. When synaptosomes were incubated with PC liposomes labeled by either rhodamine B or calcein as a fluorescence probe, or 12-doxyl stearic acid as an ESR spin trapping agent, translocation of each probe into oxidatively damaged pre-synaptic membranes was decreased significantly. Fatty acid composition analysis in pre-synaptic membranes obtained from normal rats revealed a marked increase in linoleic acid and a moderate decrease in docosahexaenoic content after the incubation with liposomes. However, rats subjected to hyperoxia did not show marked changes in these fatty acid contents in their pre-synaptic membranes after the incubation. Such changes caused by hyperoxia were inhibited by vitamin E treatment of rats. These results suggest that oxidative damage of pre-synaptic membranes caused by oxidative stress lowers the lipid-mixing for the membrane fusion. The results of this study imply that vitamin E prevents the deficit in neurotransmission at nerve terminals due to the decline in fusion between pre-synaptic membrane and synaptic vesicles caused by oxidative membrane damage.",
keywords = "Lipid-mixing, Neurotransmission, Oxidative damage, Synaptic membrane fusion, Vitamin E",
author = "Omoi, {Nao Omi} and Masaya Arai and Mitsunari Saito and Hirokatsu Takatsu and Akira Shibata and Kenji Fukuzawa and Kazue Sato and Kouichi Abe and Koji Fukui and Shiro Urano",
year = "2006",
month = "8",
language = "English",
volume = "52",
pages = "248--255",
journal = "Journal of Nutritional Science and Vitaminology",
issn = "0301-4800",
publisher = "Center for Academic Publications Japan",
number = "4",

}

TY - JOUR

T1 - Influence of oxidative stress on fusion of pre-synaptic plasma membranes of the rat brain with phosphatidyl choline liposomes, and protective effect of vitamin E

AU - Omoi, Nao Omi

AU - Arai, Masaya

AU - Saito, Mitsunari

AU - Takatsu, Hirokatsu

AU - Shibata, Akira

AU - Fukuzawa, Kenji

AU - Sato, Kazue

AU - Abe, Kouichi

AU - Fukui, Koji

AU - Urano, Shiro

PY - 2006/8

Y1 - 2006/8

N2 - Influence of oxidative stress on fusion of pre-synaptic plasma membranes with phosphatidylcholine (PC) liposomes as a model of synaptic vesicle was investigated. The inhibitory effect of vitamin E on the decline in the fusion caused by oxidative stress was also assessed. Rats subjected to hyperoxia as oxidative stress showed significant increases in the levels of lipid hydroperoxides and protein carbonyl moieties in pre-synaptic plasma membranes in the brain. The ζ potential of pre-synaptic membrane surface was decreased markedly. When synaptosomes were incubated with PC liposomes labeled by either rhodamine B or calcein as a fluorescence probe, or 12-doxyl stearic acid as an ESR spin trapping agent, translocation of each probe into oxidatively damaged pre-synaptic membranes was decreased significantly. Fatty acid composition analysis in pre-synaptic membranes obtained from normal rats revealed a marked increase in linoleic acid and a moderate decrease in docosahexaenoic content after the incubation with liposomes. However, rats subjected to hyperoxia did not show marked changes in these fatty acid contents in their pre-synaptic membranes after the incubation. Such changes caused by hyperoxia were inhibited by vitamin E treatment of rats. These results suggest that oxidative damage of pre-synaptic membranes caused by oxidative stress lowers the lipid-mixing for the membrane fusion. The results of this study imply that vitamin E prevents the deficit in neurotransmission at nerve terminals due to the decline in fusion between pre-synaptic membrane and synaptic vesicles caused by oxidative membrane damage.

AB - Influence of oxidative stress on fusion of pre-synaptic plasma membranes with phosphatidylcholine (PC) liposomes as a model of synaptic vesicle was investigated. The inhibitory effect of vitamin E on the decline in the fusion caused by oxidative stress was also assessed. Rats subjected to hyperoxia as oxidative stress showed significant increases in the levels of lipid hydroperoxides and protein carbonyl moieties in pre-synaptic plasma membranes in the brain. The ζ potential of pre-synaptic membrane surface was decreased markedly. When synaptosomes were incubated with PC liposomes labeled by either rhodamine B or calcein as a fluorescence probe, or 12-doxyl stearic acid as an ESR spin trapping agent, translocation of each probe into oxidatively damaged pre-synaptic membranes was decreased significantly. Fatty acid composition analysis in pre-synaptic membranes obtained from normal rats revealed a marked increase in linoleic acid and a moderate decrease in docosahexaenoic content after the incubation with liposomes. However, rats subjected to hyperoxia did not show marked changes in these fatty acid contents in their pre-synaptic membranes after the incubation. Such changes caused by hyperoxia were inhibited by vitamin E treatment of rats. These results suggest that oxidative damage of pre-synaptic membranes caused by oxidative stress lowers the lipid-mixing for the membrane fusion. The results of this study imply that vitamin E prevents the deficit in neurotransmission at nerve terminals due to the decline in fusion between pre-synaptic membrane and synaptic vesicles caused by oxidative membrane damage.

KW - Lipid-mixing

KW - Neurotransmission

KW - Oxidative damage

KW - Synaptic membrane fusion

KW - Vitamin E

UR - http://www.scopus.com/inward/record.url?scp=33749472529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749472529&partnerID=8YFLogxK

M3 - Article

VL - 52

SP - 248

EP - 255

JO - Journal of Nutritional Science and Vitaminology

JF - Journal of Nutritional Science and Vitaminology

SN - 0301-4800

IS - 4

ER -