Influence of the solvent on nature of gate effect in molecularly imprinted membrane

Yasuo Yoshimi, Rika Arai, Satomi Nakayama

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The solute diffusive permeability in a thin layer of a molecularly imprinted polymer (MIP) is affected by specific binding of the MIP with a template. This phenomenon, termed the " gate effect," would be widely applicable for the development of novel biomimetic sensors. However, the mechanism underlying the gate effect is not totally understood. We present here investigation of the role of specific adsorption of a template and solution content in MIPs on the gate effect. A molecularly imprinted self-supporting membrane was formed by copolymerization of methacrylic acid, 2-vinylpyridine, and triethyleneglycol dimethacrylate in the presence of l- (or d-) phenylalanine as a template. The template adsorbed by membrane with degree of enantio-selectivity in a mixed solvent of methanol and water. The amount of adsorption and binding selectivity showed little sensitivity to the solvent composition. The solution content in the membrane increased with increasing the methanol concentration of the solvent following a sigmoid curve with an inflection point at methanol concentration of 20. wt.%. The content increased in the presence of the template at methanol concentrations higher than the inflection point, and decreased at lower methanol concentrations. The creatinine permeability across the membrane estimated by batchwise dialysis increased in the presence of the template at 50. wt.% methanol in the solvent, and did not change at 20. wt.%. There was no permeability for creatinine in the pure water solvent. Both the solution content and the permeability were not affected by the presence of the enantiomer of the template. The results show that the choice of solvent controls more strongly the nature of the gate effect than the specific binding of the template.

Original languageEnglish
Pages (from-to)110-116
Number of pages7
JournalAnalytica Chimica Acta
Volume682
Issue number1-2
DOIs
Publication statusPublished - 2010 Dec 3

Fingerprint

Methanol
methanol
membrane
Membranes
permeability
Creatinine
Polymers
polymer
adsorption
Adsorption
Dialysis
Enantiomers
Water
Enantioselectivity
Biomimetics
Phenylalanine
Copolymerization
effect
solute
sensor

Keywords

  • Adsorption
  • Chirality
  • Gate effect
  • Molecularly imprinted polymer
  • Permeability
  • Solution content

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Influence of the solvent on nature of gate effect in molecularly imprinted membrane. / Yoshimi, Yasuo; Arai, Rika; Nakayama, Satomi.

In: Analytica Chimica Acta, Vol. 682, No. 1-2, 03.12.2010, p. 110-116.

Research output: Contribution to journalArticle

Yoshimi, Yasuo ; Arai, Rika ; Nakayama, Satomi. / Influence of the solvent on nature of gate effect in molecularly imprinted membrane. In: Analytica Chimica Acta. 2010 ; Vol. 682, No. 1-2. pp. 110-116.
@article{acc1cb0378b64786a8ec87e45d54d9a7,
title = "Influence of the solvent on nature of gate effect in molecularly imprinted membrane",
abstract = "The solute diffusive permeability in a thin layer of a molecularly imprinted polymer (MIP) is affected by specific binding of the MIP with a template. This phenomenon, termed the {"} gate effect,{"} would be widely applicable for the development of novel biomimetic sensors. However, the mechanism underlying the gate effect is not totally understood. We present here investigation of the role of specific adsorption of a template and solution content in MIPs on the gate effect. A molecularly imprinted self-supporting membrane was formed by copolymerization of methacrylic acid, 2-vinylpyridine, and triethyleneglycol dimethacrylate in the presence of l- (or d-) phenylalanine as a template. The template adsorbed by membrane with degree of enantio-selectivity in a mixed solvent of methanol and water. The amount of adsorption and binding selectivity showed little sensitivity to the solvent composition. The solution content in the membrane increased with increasing the methanol concentration of the solvent following a sigmoid curve with an inflection point at methanol concentration of 20. wt.{\%}. The content increased in the presence of the template at methanol concentrations higher than the inflection point, and decreased at lower methanol concentrations. The creatinine permeability across the membrane estimated by batchwise dialysis increased in the presence of the template at 50. wt.{\%} methanol in the solvent, and did not change at 20. wt.{\%}. There was no permeability for creatinine in the pure water solvent. Both the solution content and the permeability were not affected by the presence of the enantiomer of the template. The results show that the choice of solvent controls more strongly the nature of the gate effect than the specific binding of the template.",
keywords = "Adsorption, Chirality, Gate effect, Molecularly imprinted polymer, Permeability, Solution content",
author = "Yasuo Yoshimi and Rika Arai and Satomi Nakayama",
year = "2010",
month = "12",
day = "3",
doi = "10.1016/j.aca.2010.09.050",
language = "English",
volume = "682",
pages = "110--116",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Influence of the solvent on nature of gate effect in molecularly imprinted membrane

AU - Yoshimi, Yasuo

AU - Arai, Rika

AU - Nakayama, Satomi

PY - 2010/12/3

Y1 - 2010/12/3

N2 - The solute diffusive permeability in a thin layer of a molecularly imprinted polymer (MIP) is affected by specific binding of the MIP with a template. This phenomenon, termed the " gate effect," would be widely applicable for the development of novel biomimetic sensors. However, the mechanism underlying the gate effect is not totally understood. We present here investigation of the role of specific adsorption of a template and solution content in MIPs on the gate effect. A molecularly imprinted self-supporting membrane was formed by copolymerization of methacrylic acid, 2-vinylpyridine, and triethyleneglycol dimethacrylate in the presence of l- (or d-) phenylalanine as a template. The template adsorbed by membrane with degree of enantio-selectivity in a mixed solvent of methanol and water. The amount of adsorption and binding selectivity showed little sensitivity to the solvent composition. The solution content in the membrane increased with increasing the methanol concentration of the solvent following a sigmoid curve with an inflection point at methanol concentration of 20. wt.%. The content increased in the presence of the template at methanol concentrations higher than the inflection point, and decreased at lower methanol concentrations. The creatinine permeability across the membrane estimated by batchwise dialysis increased in the presence of the template at 50. wt.% methanol in the solvent, and did not change at 20. wt.%. There was no permeability for creatinine in the pure water solvent. Both the solution content and the permeability were not affected by the presence of the enantiomer of the template. The results show that the choice of solvent controls more strongly the nature of the gate effect than the specific binding of the template.

AB - The solute diffusive permeability in a thin layer of a molecularly imprinted polymer (MIP) is affected by specific binding of the MIP with a template. This phenomenon, termed the " gate effect," would be widely applicable for the development of novel biomimetic sensors. However, the mechanism underlying the gate effect is not totally understood. We present here investigation of the role of specific adsorption of a template and solution content in MIPs on the gate effect. A molecularly imprinted self-supporting membrane was formed by copolymerization of methacrylic acid, 2-vinylpyridine, and triethyleneglycol dimethacrylate in the presence of l- (or d-) phenylalanine as a template. The template adsorbed by membrane with degree of enantio-selectivity in a mixed solvent of methanol and water. The amount of adsorption and binding selectivity showed little sensitivity to the solvent composition. The solution content in the membrane increased with increasing the methanol concentration of the solvent following a sigmoid curve with an inflection point at methanol concentration of 20. wt.%. The content increased in the presence of the template at methanol concentrations higher than the inflection point, and decreased at lower methanol concentrations. The creatinine permeability across the membrane estimated by batchwise dialysis increased in the presence of the template at 50. wt.% methanol in the solvent, and did not change at 20. wt.%. There was no permeability for creatinine in the pure water solvent. Both the solution content and the permeability were not affected by the presence of the enantiomer of the template. The results show that the choice of solvent controls more strongly the nature of the gate effect than the specific binding of the template.

KW - Adsorption

KW - Chirality

KW - Gate effect

KW - Molecularly imprinted polymer

KW - Permeability

KW - Solution content

UR - http://www.scopus.com/inward/record.url?scp=78149406546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78149406546&partnerID=8YFLogxK

U2 - 10.1016/j.aca.2010.09.050

DO - 10.1016/j.aca.2010.09.050

M3 - Article

C2 - 21056722

AN - SCOPUS:78149406546

VL - 682

SP - 110

EP - 116

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 1-2

ER -