Ingestion of cocoa ameliorates endothelial dysfunction in mesentery arterioles induced by high fat diet in rats: An in vivo intravital microscopy study

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aims:Numerous clinical studies have reported that ingestion of cocoa has a therapeutic effect on hypertension. However, there is only limited information on the mechanism of ingestion of cocoa on arterioles, vessels that have a major role in determining blood pressure. In this study, we used intravital video-microscopy to evaluate the effect of cocoa consumption on the mesentery microcirculation of rats fed a high fat diet. Main methods:The animals were allocated to 3 groups, and fed either a control diet, a high fat diet containing 15% lard, or the HFD with 2% cocoa (HFD-C) for 6 weeks. At the end of the experimental period, the mesentery was spread in a chamber, and the vessels were treated topically with phenylephrine, acetylcholine, or papaverine. The vascular responses to phenylephrine, acetylcholine-dependent vasodilatation and endothelium-independent vasodilatation were calculated by the diameter of the mesentery artery with each treatment. Key findings:Topical treatment of mesenteric arterioles with acetylcholine caused a significantly greater response in the control compared with the HFD group. In the HFD-C group, acetylcholine-dependent vasodilatation was decreased marginally. Similarly, rats in the HFD group showed a significant reduction in vascular sensitivity to phenylephrine compared with the control group. However, there was no significant difference between the control and HFD-C groups. The induction of endothelial-independent artery dilation was reduced slightly in the HFD group. Significance:Our results suggest that one of the hypotensive mechanisms of cocoa is due to amelioration of endothelial dysfunction in arterioles induced by an inappropriate diet.

Original languageEnglish
Pages (from-to)1196-1200
Number of pages5
JournalLife Sciences
Volume91
Issue number23-24
DOIs
Publication statusPublished - 2012 Dec 10

Fingerprint

Cocoa
Nutrition
Rats
Fats
Acetylcholine
Phenylephrine
Microcirculation
Papaverine
Blood pressure
Intravital Microscopy
Microscopic examination
Animals

Keywords

  • Cocoa
  • Intravital microscopy
  • Mesentery
  • Rat

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{05685a6047d44e6ba4d9993edec8a725,
title = "Ingestion of cocoa ameliorates endothelial dysfunction in mesentery arterioles induced by high fat diet in rats: An in vivo intravital microscopy study",
abstract = "Aims:Numerous clinical studies have reported that ingestion of cocoa has a therapeutic effect on hypertension. However, there is only limited information on the mechanism of ingestion of cocoa on arterioles, vessels that have a major role in determining blood pressure. In this study, we used intravital video-microscopy to evaluate the effect of cocoa consumption on the mesentery microcirculation of rats fed a high fat diet. Main methods:The animals were allocated to 3 groups, and fed either a control diet, a high fat diet containing 15{\%} lard, or the HFD with 2{\%} cocoa (HFD-C) for 6 weeks. At the end of the experimental period, the mesentery was spread in a chamber, and the vessels were treated topically with phenylephrine, acetylcholine, or papaverine. The vascular responses to phenylephrine, acetylcholine-dependent vasodilatation and endothelium-independent vasodilatation were calculated by the diameter of the mesentery artery with each treatment. Key findings:Topical treatment of mesenteric arterioles with acetylcholine caused a significantly greater response in the control compared with the HFD group. In the HFD-C group, acetylcholine-dependent vasodilatation was decreased marginally. Similarly, rats in the HFD group showed a significant reduction in vascular sensitivity to phenylephrine compared with the control group. However, there was no significant difference between the control and HFD-C groups. The induction of endothelial-independent artery dilation was reduced slightly in the HFD group. Significance:Our results suggest that one of the hypotensive mechanisms of cocoa is due to amelioration of endothelial dysfunction in arterioles induced by an inappropriate diet.",
keywords = "Cocoa, Intravital microscopy, Mesentery, Rat",
author = "Naomi Osakabe and Masahiro Shibata",
year = "2012",
month = "12",
day = "10",
doi = "10.1016/j.lfs.2012.08.031",
language = "English",
volume = "91",
pages = "1196--1200",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "23-24",

}

TY - JOUR

T1 - Ingestion of cocoa ameliorates endothelial dysfunction in mesentery arterioles induced by high fat diet in rats

T2 - An in vivo intravital microscopy study

AU - Osakabe, Naomi

AU - Shibata, Masahiro

PY - 2012/12/10

Y1 - 2012/12/10

N2 - Aims:Numerous clinical studies have reported that ingestion of cocoa has a therapeutic effect on hypertension. However, there is only limited information on the mechanism of ingestion of cocoa on arterioles, vessels that have a major role in determining blood pressure. In this study, we used intravital video-microscopy to evaluate the effect of cocoa consumption on the mesentery microcirculation of rats fed a high fat diet. Main methods:The animals were allocated to 3 groups, and fed either a control diet, a high fat diet containing 15% lard, or the HFD with 2% cocoa (HFD-C) for 6 weeks. At the end of the experimental period, the mesentery was spread in a chamber, and the vessels were treated topically with phenylephrine, acetylcholine, or papaverine. The vascular responses to phenylephrine, acetylcholine-dependent vasodilatation and endothelium-independent vasodilatation were calculated by the diameter of the mesentery artery with each treatment. Key findings:Topical treatment of mesenteric arterioles with acetylcholine caused a significantly greater response in the control compared with the HFD group. In the HFD-C group, acetylcholine-dependent vasodilatation was decreased marginally. Similarly, rats in the HFD group showed a significant reduction in vascular sensitivity to phenylephrine compared with the control group. However, there was no significant difference between the control and HFD-C groups. The induction of endothelial-independent artery dilation was reduced slightly in the HFD group. Significance:Our results suggest that one of the hypotensive mechanisms of cocoa is due to amelioration of endothelial dysfunction in arterioles induced by an inappropriate diet.

AB - Aims:Numerous clinical studies have reported that ingestion of cocoa has a therapeutic effect on hypertension. However, there is only limited information on the mechanism of ingestion of cocoa on arterioles, vessels that have a major role in determining blood pressure. In this study, we used intravital video-microscopy to evaluate the effect of cocoa consumption on the mesentery microcirculation of rats fed a high fat diet. Main methods:The animals were allocated to 3 groups, and fed either a control diet, a high fat diet containing 15% lard, or the HFD with 2% cocoa (HFD-C) for 6 weeks. At the end of the experimental period, the mesentery was spread in a chamber, and the vessels were treated topically with phenylephrine, acetylcholine, or papaverine. The vascular responses to phenylephrine, acetylcholine-dependent vasodilatation and endothelium-independent vasodilatation were calculated by the diameter of the mesentery artery with each treatment. Key findings:Topical treatment of mesenteric arterioles with acetylcholine caused a significantly greater response in the control compared with the HFD group. In the HFD-C group, acetylcholine-dependent vasodilatation was decreased marginally. Similarly, rats in the HFD group showed a significant reduction in vascular sensitivity to phenylephrine compared with the control group. However, there was no significant difference between the control and HFD-C groups. The induction of endothelial-independent artery dilation was reduced slightly in the HFD group. Significance:Our results suggest that one of the hypotensive mechanisms of cocoa is due to amelioration of endothelial dysfunction in arterioles induced by an inappropriate diet.

KW - Cocoa

KW - Intravital microscopy

KW - Mesentery

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=84869490632&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84869490632&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2012.08.031

DO - 10.1016/j.lfs.2012.08.031

M3 - Article

C2 - 22982348

AN - SCOPUS:84869490632

VL - 91

SP - 1196

EP - 1200

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 23-24

ER -