Integrated on-chip 3D vascular network culture under hypoxia

Miguel Ángel Olmedo-Suárez, Tomohiro Sekiguchi, Atsushi Takano, Maria del Pilar Cañizares-Macías, Nobuyuki Futai

Research output: Contribution to journalArticle

Abstract

We developed a portable device made of poly(dimethylsiloxane) (PDMS)/polymethylmethacrylate (PMMA) for long-term 3D cell culture of vascular endothelial cells for the development of a vascular network and evaluated the device under different transitions between normoxia and hypoxia with good optical accessibility. The combination of a nested reservoir device and a bicarbonate/ascorbate buffer system accomplished on-chip incubation with 4.91 ± 0.86% pO2 and 5.19 ± 1.70% pCO2 for up to 10 days. Seventy-two hours of normoxic incubation preceding hypoxic culture increased the cell viability, network formation, and size and stability of the resulting lumens compared with those completely maintained in normoxia for the same total duration. We employed different parameters of the network (e.g., total mesh area, total length, number of branches, among others) for the comparison of different oxygen treatments in the device. The differential effect of hypoxic conditions based on the maturity of the vessels may be used as an external factor to improve vascular development in vitro.

Original languageEnglish
Article number475
JournalMicromachines
Volume11
Issue number5
DOIs
Publication statusPublished - 2020 May 1

Keywords

  • 3D cell culture
  • Angiogenesis
  • Hypoxia
  • Normoxic/hypoxic transition
  • Portable cell culture device
  • Vascular network

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Olmedo-Suárez, M. Á., Sekiguchi, T., Takano, A., Cañizares-Macías, M. D. P., & Futai, N. (2020). Integrated on-chip 3D vascular network culture under hypoxia. Micromachines, 11(5), [475]. https://doi.org/10.3390/MI11050475