Interactions of arsenic with human metallothionein-2

Mitsutoshi Toyama; Mitsuo Yamashita; Noriaki Hirayama; Yoshikatsu Murooka

doi:10.1093/oxfordjournals.jbchem.a003213

Interactions of arsenic with human metallothionein-2

Mitsutoshi Toyama, Mitsuo Yamashita, Noriaki Hirayama, Yoshikatsu Murooka

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Arsenic is a toxic element that is found in the atmosphere, as well as in aquatic and terrestrial environments. We have demonstrated that As³⁺ binds to human metallothionein-2 (hMT-2) by UV absorption spectroscopy, inductively coupled plasma-atomic emission spectrometry (ICP-AES), and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-TOF-MS revealed that the structure of the adduct formed by arsenic and hMT-2 (As-hMT-2) was not homogeneous. The maximum molar ratio of arsenic to hMT-2 was found to be more than 6:1 on ICP-AES, UV absorption spectroscopy and MALDI-TOF-MS. The ratio of the number of sulfhydryl groups in hMT-2 that bound arsenic was 3:1, which is the same as the ratios reported previously for arsenic-glutathione and arsenic-phytochelatin complexes.

Original language	English
Pages (from-to)	217-221
Number of pages	5
Journal	Journal of biochemistry
Volume	132
Issue number	2
DOIs	https://doi.org/10.1093/oxfordjournals.jbchem.a003213
Publication status	Published - 2002 Aug
Externally published	Yes

Keywords

Arsenic
Heavy metal binding protein
Human metallothionein
MALDI-TOF-MS

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1093/oxfordjournals.jbchem.a003213

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title = "Interactions of arsenic with human metallothionein-2",

abstract = "Arsenic is a toxic element that is found in the atmosphere, as well as in aquatic and terrestrial environments. We have demonstrated that As3+ binds to human metallothionein-2 (hMT-2) by UV absorption spectroscopy, inductively coupled plasma-atomic emission spectrometry (ICP-AES), and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-TOF-MS revealed that the structure of the adduct formed by arsenic and hMT-2 (As-hMT-2) was not homogeneous. The maximum molar ratio of arsenic to hMT-2 was found to be more than 6:1 on ICP-AES, UV absorption spectroscopy and MALDI-TOF-MS. The ratio of the number of sulfhydryl groups in hMT-2 that bound arsenic was 3:1, which is the same as the ratios reported previously for arsenic-glutathione and arsenic-phytochelatin complexes.",

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author = "Mitsutoshi Toyama and Mitsuo Yamashita and Noriaki Hirayama and Yoshikatsu Murooka",

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T1 - Interactions of arsenic with human metallothionein-2

AU - Toyama, Mitsutoshi

AU - Yamashita, Mitsuo

AU - Hirayama, Noriaki

AU - Murooka, Yoshikatsu

PY - 2002/8

Y1 - 2002/8

N2 - Arsenic is a toxic element that is found in the atmosphere, as well as in aquatic and terrestrial environments. We have demonstrated that As3+ binds to human metallothionein-2 (hMT-2) by UV absorption spectroscopy, inductively coupled plasma-atomic emission spectrometry (ICP-AES), and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-TOF-MS revealed that the structure of the adduct formed by arsenic and hMT-2 (As-hMT-2) was not homogeneous. The maximum molar ratio of arsenic to hMT-2 was found to be more than 6:1 on ICP-AES, UV absorption spectroscopy and MALDI-TOF-MS. The ratio of the number of sulfhydryl groups in hMT-2 that bound arsenic was 3:1, which is the same as the ratios reported previously for arsenic-glutathione and arsenic-phytochelatin complexes.

AB - Arsenic is a toxic element that is found in the atmosphere, as well as in aquatic and terrestrial environments. We have demonstrated that As3+ binds to human metallothionein-2 (hMT-2) by UV absorption spectroscopy, inductively coupled plasma-atomic emission spectrometry (ICP-AES), and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-TOF-MS revealed that the structure of the adduct formed by arsenic and hMT-2 (As-hMT-2) was not homogeneous. The maximum molar ratio of arsenic to hMT-2 was found to be more than 6:1 on ICP-AES, UV absorption spectroscopy and MALDI-TOF-MS. The ratio of the number of sulfhydryl groups in hMT-2 that bound arsenic was 3:1, which is the same as the ratios reported previously for arsenic-glutathione and arsenic-phytochelatin complexes.

KW - Arsenic

KW - Heavy metal binding protein

KW - Human metallothionein

KW - MALDI-TOF-MS

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JF - Journal of Biochemistry

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ER -

Interactions of arsenic with human metallothionein-2

Abstract

Keywords

ASJC Scopus subject areas

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