Interactions of arsenic with human metallothionein-2

Mitsutoshi Toyama, Mitsuo Yamashita, Noriaki Hirayama, Yoshikatsu Murooka

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Arsenic is a toxic element that is found in the atmosphere, as well as in aquatic and terrestrial environments. We have demonstrated that As3+ binds to human metallothionein-2 (hMT-2) by UV absorption spectroscopy, inductively coupled plasma-atomic emission spectrometry (ICP-AES), and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-TOF-MS revealed that the structure of the adduct formed by arsenic and hMT-2 (As-hMT-2) was not homogeneous. The maximum molar ratio of arsenic to hMT-2 was found to be more than 6:1 on ICP-AES, UV absorption spectroscopy and MALDI-TOF-MS. The ratio of the number of sulfhydryl groups in hMT-2 that bound arsenic was 3:1, which is the same as the ratios reported previously for arsenic-glutathione and arsenic-phytochelatin complexes.

Original languageEnglish
Pages (from-to)217-221
Number of pages5
JournalJournal of Biochemistry
Volume132
Issue number2
Publication statusPublished - 2002 Aug
Externally publishedYes

Fingerprint

Metallothionein
Arsenic
Ionization
Mass spectrometry
Desorption
Inductively coupled plasma
Ultraviolet spectroscopy
Absorption spectroscopy
Spectrometry
Lasers
Phytochelatins
Poisons
Glutathione

Keywords

  • Arsenic
  • Heavy metal binding protein
  • Human metallothionein
  • MALDI-TOF-MS

ASJC Scopus subject areas

  • Biochemistry

Cite this

Toyama, M., Yamashita, M., Hirayama, N., & Murooka, Y. (2002). Interactions of arsenic with human metallothionein-2. Journal of Biochemistry, 132(2), 217-221.

Interactions of arsenic with human metallothionein-2. / Toyama, Mitsutoshi; Yamashita, Mitsuo; Hirayama, Noriaki; Murooka, Yoshikatsu.

In: Journal of Biochemistry, Vol. 132, No. 2, 08.2002, p. 217-221.

Research output: Contribution to journalArticle

Toyama, M, Yamashita, M, Hirayama, N & Murooka, Y 2002, 'Interactions of arsenic with human metallothionein-2', Journal of Biochemistry, vol. 132, no. 2, pp. 217-221.
Toyama M, Yamashita M, Hirayama N, Murooka Y. Interactions of arsenic with human metallothionein-2. Journal of Biochemistry. 2002 Aug;132(2):217-221.
Toyama, Mitsutoshi ; Yamashita, Mitsuo ; Hirayama, Noriaki ; Murooka, Yoshikatsu. / Interactions of arsenic with human metallothionein-2. In: Journal of Biochemistry. 2002 ; Vol. 132, No. 2. pp. 217-221.
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