Irradiation of Fucci-expressing HeLa cells using a tapered glass capillary microbeam

Nitipon Puttaraksa, Volkhard Mäckel, Tomohiro Kobayashi, Takao M. Kojima, Manabu Hamagaki, Naoko Imamoto, Yasunori Yamazaki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Fluorescent, ubiquitination-based cell cycle indicator-expressing HeLa cells have been irradiated with a microbeam set-up at RIKEN for examining the effect to cell cycle after bombarding with protons of 1 MeV incident energy. Here, single cells were exposed in their nucleus with different doses (100, 1000, 10,000 and 100,000 protons). Subsequently, the cells were monitored using time-lapse microscopy after irradiation. In this paper, we present the preliminary results for such irradiation for 8 cells. The irradiated cells with up to 10,000 protons showed the change in fluorescence from red to green. This indicates that the transformation from G1 to S/G2 in cell cycle seemed not to be affected by the irradiation up to 10,000 protons. However, the irradiated cell with 100,000 protons died in red fluorescence at ∼4 h after the irradiation.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Publication statusPublished - 2015
Externally publishedYes


  • Cell irradiation
  • Fucci-expressing HeLa cells
  • Microbeam
  • Tapered glass capillary

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Instrumentation


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