TY - JOUR
T1 - Irradiation of Fucci-expressing HeLa cells using a tapered glass capillary microbeam
AU - Puttaraksa, Nitipon
AU - Mäckel, Volkhard
AU - Kobayashi, Tomohiro
AU - Kojima, Takao M.
AU - Hamagaki, Manabu
AU - Imamoto, Naoko
AU - Yamazaki, Yasunori
N1 - Funding Information:
We gratefully acknowledge Dr. Atsushi Miyawaki for providing the Fucci-expressing HeLa cells. We would like to thank RIKEN BSI-Olympus Collaboration Center for technical support. This work was supported by a grant of Special Projects for Basic Science of RIKEN “Pursuing a new science field using extreme particle beams”.
Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.
PY - 2015
Y1 - 2015
N2 - Fluorescent, ubiquitination-based cell cycle indicator-expressing HeLa cells have been irradiated with a microbeam set-up at RIKEN for examining the effect to cell cycle after bombarding with protons of 1 MeV incident energy. Here, single cells were exposed in their nucleus with different doses (100, 1000, 10,000 and 100,000 protons). Subsequently, the cells were monitored using time-lapse microscopy after irradiation. In this paper, we present the preliminary results for such irradiation for 8 cells. The irradiated cells with up to 10,000 protons showed the change in fluorescence from red to green. This indicates that the transformation from G1 to S/G2 in cell cycle seemed not to be affected by the irradiation up to 10,000 protons. However, the irradiated cell with 100,000 protons died in red fluorescence at ∼4 h after the irradiation.
AB - Fluorescent, ubiquitination-based cell cycle indicator-expressing HeLa cells have been irradiated with a microbeam set-up at RIKEN for examining the effect to cell cycle after bombarding with protons of 1 MeV incident energy. Here, single cells were exposed in their nucleus with different doses (100, 1000, 10,000 and 100,000 protons). Subsequently, the cells were monitored using time-lapse microscopy after irradiation. In this paper, we present the preliminary results for such irradiation for 8 cells. The irradiated cells with up to 10,000 protons showed the change in fluorescence from red to green. This indicates that the transformation from G1 to S/G2 in cell cycle seemed not to be affected by the irradiation up to 10,000 protons. However, the irradiated cell with 100,000 protons died in red fluorescence at ∼4 h after the irradiation.
KW - Cell irradiation
KW - Fucci-expressing HeLa cells
KW - Microbeam
KW - Tapered glass capillary
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U2 - 10.1016/j.nimb.2014.11.037
DO - 10.1016/j.nimb.2014.11.037
M3 - Article
AN - SCOPUS:84930965839
SN - 0168-583X
VL - 348
SP - 127
EP - 130
JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
ER -