Isolation and characterization of a car gene cluster from the naphthalene, phenanthrene, and carbazole-degrading marine isolate Lysobacter sp. strain OC7

Rintaro Maeda, Hiroshi Nagashima, Azham Bin Zulkharnain, Kenichi Iwata, Toshio Omori

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The novel carbazole (CAR)-degrading bacterium Lysobacter sp. strain OC7 has been isolated from seawater and can also utilize naphthalene and phenanthrene as its sole carbon and energy source. The CAR-degradative gene cluster was isolated and encoded five complete open reading frames (ORFs) and two truncated ORFs. Among them, four ORFs showed 40-50% similarity with previously reported CAR-degradative genes. Ferredoxin (carAc) and ferredoxin reductase (carAd) genes, which are necessary for the CAR 1,9a-dioxygenase system, were not found in this car gene cluster. The car OC7 gene transcripts were strongly detected when CAR was provided. However, these transcripts were also detected when naphthalene was provided. The resting cell reaction with Escherichia coli revealed that CarAaOC7 can use CarAc and CarAd of Pseudomonas resinovorans CA10 as ferredoxin and ferredoxin reductase, respectively, and converted CAR to 2′-aminobiphenyl-2,3-diol. In 13 marine CAR-degrading isolates, only Caulobacter sp. strain OC6 hybridized with the car OC7 gene cluster probe. This is the first report showing CAR-degradative genes from the genus Lysobacter.

Original languageEnglish
Pages (from-to)154-159
Number of pages6
JournalCurrent Microbiology
Volume59
Issue number2
DOIs
Publication statusPublished - 2009 Aug

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Isolation and characterization of a car gene cluster from the naphthalene, phenanthrene, and carbazole-degrading marine isolate Lysobacter sp. strain OC7. / Maeda, Rintaro; Nagashima, Hiroshi; Bin Zulkharnain, Azham; Iwata, Kenichi; Omori, Toshio.

In: Current Microbiology, Vol. 59, No. 2, 08.2009, p. 154-159.

Research output: Contribution to journalArticle

@article{a12083e5f8034f5a8384ad1b5ab42f7e,
title = "Isolation and characterization of a car gene cluster from the naphthalene, phenanthrene, and carbazole-degrading marine isolate Lysobacter sp. strain OC7",
abstract = "The novel carbazole (CAR)-degrading bacterium Lysobacter sp. strain OC7 has been isolated from seawater and can also utilize naphthalene and phenanthrene as its sole carbon and energy source. The CAR-degradative gene cluster was isolated and encoded five complete open reading frames (ORFs) and two truncated ORFs. Among them, four ORFs showed 40-50{\%} similarity with previously reported CAR-degradative genes. Ferredoxin (carAc) and ferredoxin reductase (carAd) genes, which are necessary for the CAR 1,9a-dioxygenase system, were not found in this car gene cluster. The car OC7 gene transcripts were strongly detected when CAR was provided. However, these transcripts were also detected when naphthalene was provided. The resting cell reaction with Escherichia coli revealed that CarAaOC7 can use CarAc and CarAd of Pseudomonas resinovorans CA10 as ferredoxin and ferredoxin reductase, respectively, and converted CAR to 2′-aminobiphenyl-2,3-diol. In 13 marine CAR-degrading isolates, only Caulobacter sp. strain OC6 hybridized with the car OC7 gene cluster probe. This is the first report showing CAR-degradative genes from the genus Lysobacter.",
author = "Rintaro Maeda and Hiroshi Nagashima and {Bin Zulkharnain}, Azham and Kenichi Iwata and Toshio Omori",
year = "2009",
month = "8",
doi = "10.1007/s00284-009-9414-y",
language = "English",
volume = "59",
pages = "154--159",
journal = "Current Microbiology",
issn = "0343-8651",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Isolation and characterization of a car gene cluster from the naphthalene, phenanthrene, and carbazole-degrading marine isolate Lysobacter sp. strain OC7

AU - Maeda, Rintaro

AU - Nagashima, Hiroshi

AU - Bin Zulkharnain, Azham

AU - Iwata, Kenichi

AU - Omori, Toshio

PY - 2009/8

Y1 - 2009/8

N2 - The novel carbazole (CAR)-degrading bacterium Lysobacter sp. strain OC7 has been isolated from seawater and can also utilize naphthalene and phenanthrene as its sole carbon and energy source. The CAR-degradative gene cluster was isolated and encoded five complete open reading frames (ORFs) and two truncated ORFs. Among them, four ORFs showed 40-50% similarity with previously reported CAR-degradative genes. Ferredoxin (carAc) and ferredoxin reductase (carAd) genes, which are necessary for the CAR 1,9a-dioxygenase system, were not found in this car gene cluster. The car OC7 gene transcripts were strongly detected when CAR was provided. However, these transcripts were also detected when naphthalene was provided. The resting cell reaction with Escherichia coli revealed that CarAaOC7 can use CarAc and CarAd of Pseudomonas resinovorans CA10 as ferredoxin and ferredoxin reductase, respectively, and converted CAR to 2′-aminobiphenyl-2,3-diol. In 13 marine CAR-degrading isolates, only Caulobacter sp. strain OC6 hybridized with the car OC7 gene cluster probe. This is the first report showing CAR-degradative genes from the genus Lysobacter.

AB - The novel carbazole (CAR)-degrading bacterium Lysobacter sp. strain OC7 has been isolated from seawater and can also utilize naphthalene and phenanthrene as its sole carbon and energy source. The CAR-degradative gene cluster was isolated and encoded five complete open reading frames (ORFs) and two truncated ORFs. Among them, four ORFs showed 40-50% similarity with previously reported CAR-degradative genes. Ferredoxin (carAc) and ferredoxin reductase (carAd) genes, which are necessary for the CAR 1,9a-dioxygenase system, were not found in this car gene cluster. The car OC7 gene transcripts were strongly detected when CAR was provided. However, these transcripts were also detected when naphthalene was provided. The resting cell reaction with Escherichia coli revealed that CarAaOC7 can use CarAc and CarAd of Pseudomonas resinovorans CA10 as ferredoxin and ferredoxin reductase, respectively, and converted CAR to 2′-aminobiphenyl-2,3-diol. In 13 marine CAR-degrading isolates, only Caulobacter sp. strain OC6 hybridized with the car OC7 gene cluster probe. This is the first report showing CAR-degradative genes from the genus Lysobacter.

UR - http://www.scopus.com/inward/record.url?scp=67651166633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67651166633&partnerID=8YFLogxK

U2 - 10.1007/s00284-009-9414-y

DO - 10.1007/s00284-009-9414-y

M3 - Article

C2 - 19452212

AN - SCOPUS:67651166633

VL - 59

SP - 154

EP - 159

JO - Current Microbiology

JF - Current Microbiology

SN - 0343-8651

IS - 2

ER -