Isolation and characterization of a mouse SRY-related cDNA, mSox7

Koji Taniguchi, Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Susumu Kido, Sadakazu Aiso

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1445
Issue number2
DOIs
Publication statusPublished - 1999 May 14
Externally publishedYes

Fingerprint

Complementary DNA
Genes
Electrophoretic mobility
Assays
Nucleotides
Tissue
Amino Acids
Peptides
Proteins

Keywords

  • DNA binding
  • HMG box
  • Oocyte
  • SOX
  • SRY

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Isolation and characterization of a mouse SRY-related cDNA, mSox7. / Taniguchi, Koji; Hiraoka, Yoshiki; Ogawa, Motoyuki; Sakai, Yukinao; Kido, Susumu; Aiso, Sadakazu.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1445, No. 2, 14.05.1999, p. 225-231.

Research output: Contribution to journalArticle

Taniguchi, Koji ; Hiraoka, Yoshiki ; Ogawa, Motoyuki ; Sakai, Yukinao ; Kido, Susumu ; Aiso, Sadakazu. / Isolation and characterization of a mouse SRY-related cDNA, mSox7. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 1999 ; Vol. 1445, No. 2. pp. 225-231.
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