TY - JOUR
T1 - Isolation and characterization of an alkaliphilic bacterium utilizing pyrene as a carbon source
AU - Habe, Hiroshi
AU - Kanemitsu, Mieko
AU - Nomura, Mizuki
AU - Takemura, Tetsuo
AU - Iwata, Kenichi
AU - Nojiri, Hideaki
AU - Yamane, Hisakazu
AU - Omori, Toshio
PY - 2004/10
Y1 - 2004/10
N2 - Alkaliphilic Mycobacterium sp. strain MHP-1, which can grow on pyrene as a sole carbon and energy source, was isolated from a soil sample. At the optimum pH for growth (pH 9), about 50% of pyrene (final concentration at 0.1% [w/v]) was degraded during 7 d of incubation, and 4,5-phenanthrenedioic acid, 4-phenanthroic acid and phthalic acid were identified as metabolic intermediates. Strain MHP-1 was found to possess aromatic-ring dioxygenase genes, which are highly homologous to the known nidAB genes from pyrene-degrading mycobacteria.
AB - Alkaliphilic Mycobacterium sp. strain MHP-1, which can grow on pyrene as a sole carbon and energy source, was isolated from a soil sample. At the optimum pH for growth (pH 9), about 50% of pyrene (final concentration at 0.1% [w/v]) was degraded during 7 d of incubation, and 4,5-phenanthrenedioic acid, 4-phenanthroic acid and phthalic acid were identified as metabolic intermediates. Strain MHP-1 was found to possess aromatic-ring dioxygenase genes, which are highly homologous to the known nidAB genes from pyrene-degrading mycobacteria.
KW - Alkaliphile
KW - Mycobacterium sp.
KW - Polycyclic aromatic hydrocarbons
KW - Pyrene degradation
UR - http://www.scopus.com/inward/record.url?scp=9144247768&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=9144247768&partnerID=8YFLogxK
U2 - 10.1263/jbb.98.306
DO - 10.1263/jbb.98.306
M3 - Article
C2 - 16233711
AN - SCOPUS:9144247768
SN - 1389-1723
VL - 98
SP - 306
EP - 308
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 4
ER -