Isolation and characterization of an alkaliphilic bacterium utilizing pyrene as a carbon source

Hiroshi Habe, Mieko Kanemitsu, Mizuki Nomura, Tetsuo Takemura, Kenichi Iwata, Hideaki Nojiri, Hisakazu Yamane, Toshio Omori

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Alkaliphilic Mycobacterium sp. strain MHP-1, which can grow on pyrene as a sole carbon and energy source, was isolated from a soil sample. At the optimum pH for growth (pH 9), about 50% of pyrene (final concentration at 0.1% [w/v]) was degraded during 7 d of incubation, and 4,5-phenanthrenedioic acid, 4-phenanthroic acid and phthalic acid were identified as metabolic intermediates. Strain MHP-1 was found to possess aromatic-ring dioxygenase genes, which are highly homologous to the known nidAB genes from pyrene-degrading mycobacteria.

Original languageEnglish
Pages (from-to)306-308
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume98
Issue number4
DOIs
Publication statusPublished - 2004 Oct

Keywords

  • Alkaliphile
  • Mycobacterium sp.
  • Polycyclic aromatic hydrocarbons
  • Pyrene degradation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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