Isolation and characterization of an alkaliphilic bacterium utilizing pyrene as a carbon source

Hiroshi Habe, Mieko Kanemitsu, Mizuki Nomura, Tetsuo Takemura, Kenichi Iwata, Hideaki Nojiri, Hisakazu Yamane, Toshio Omori

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Alkaliphilic Mycobacterium sp. strain MHP-1, which can grow on pyrene as a sole carbon and energy source, was isolated from a soil sample. At the optimum pH for growth (pH 9), about 50% of pyrene (final concentration at 0.1% [w/v]) was degraded during 7 d of incubation, and 4,5-phenanthrenedioic acid, 4-phenanthroic acid and phthalic acid were identified as metabolic intermediates. Strain MHP-1 was found to possess aromatic-ring dioxygenase genes, which are highly homologous to the known nidAB genes from pyrene-degrading mycobacteria.

Original languageEnglish
Pages (from-to)306-308
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume98
Issue number4
DOIs
Publication statusPublished - 2004 Oct
Externally publishedYes

Fingerprint

Pyrene
Bacteria
Carbon
Acids
Genes
Dioxygenases
Soils
pyrene

Keywords

  • Alkaliphile
  • Mycobacterium sp.
  • Polycyclic aromatic hydrocarbons
  • Pyrene degradation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Habe, H., Kanemitsu, M., Nomura, M., Takemura, T., Iwata, K., Nojiri, H., ... Omori, T. (2004). Isolation and characterization of an alkaliphilic bacterium utilizing pyrene as a carbon source. Journal of Bioscience and Bioengineering, 98(4), 306-308. https://doi.org/10.1263/jbb.98.306

Isolation and characterization of an alkaliphilic bacterium utilizing pyrene as a carbon source. / Habe, Hiroshi; Kanemitsu, Mieko; Nomura, Mizuki; Takemura, Tetsuo; Iwata, Kenichi; Nojiri, Hideaki; Yamane, Hisakazu; Omori, Toshio.

In: Journal of Bioscience and Bioengineering, Vol. 98, No. 4, 10.2004, p. 306-308.

Research output: Contribution to journalArticle

Habe, H, Kanemitsu, M, Nomura, M, Takemura, T, Iwata, K, Nojiri, H, Yamane, H & Omori, T 2004, 'Isolation and characterization of an alkaliphilic bacterium utilizing pyrene as a carbon source', Journal of Bioscience and Bioengineering, vol. 98, no. 4, pp. 306-308. https://doi.org/10.1263/jbb.98.306
Habe, Hiroshi ; Kanemitsu, Mieko ; Nomura, Mizuki ; Takemura, Tetsuo ; Iwata, Kenichi ; Nojiri, Hideaki ; Yamane, Hisakazu ; Omori, Toshio. / Isolation and characterization of an alkaliphilic bacterium utilizing pyrene as a carbon source. In: Journal of Bioscience and Bioengineering. 2004 ; Vol. 98, No. 4. pp. 306-308.
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