Isolation and characterization of Xenopus laevis xSox-B1 cDNA

Yukinao Sakai, Yoshiki Hiraoka, Masahiro Konishi, Motoyuki Ogawa, Sadakazu Aiso

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox- B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume346
Issue number1
DOIs
Publication statusPublished - 1997 Oct 1
Externally publishedYes

Fingerprint

Complementary DNA
Genes
Messenger RNA
Polymerase chain reaction
Transcription
Animals
Tissue
Amino Acids
Peptides
Proteins

Keywords

  • DNA binding
  • Embryogenesis
  • Nucleotide sequence
  • SOX
  • SRY (Xenopus laevis)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Isolation and characterization of Xenopus laevis xSox-B1 cDNA. / Sakai, Yukinao; Hiraoka, Yoshiki; Konishi, Masahiro; Ogawa, Motoyuki; Aiso, Sadakazu.

In: Archives of Biochemistry and Biophysics, Vol. 346, No. 1, 01.10.1997, p. 1-6.

Research output: Contribution to journalArticle

Sakai, Yukinao ; Hiraoka, Yoshiki ; Konishi, Masahiro ; Ogawa, Motoyuki ; Aiso, Sadakazu. / Isolation and characterization of Xenopus laevis xSox-B1 cDNA. In: Archives of Biochemistry and Biophysics. 1997 ; Vol. 346, No. 1. pp. 1-6.
@article{7d496594ddbb40589fe3d868cbffd506,
title = "Isolation and characterization of Xenopus laevis xSox-B1 cDNA",
abstract = "A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox- B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.",
keywords = "DNA binding, Embryogenesis, Nucleotide sequence, SOX, SRY (Xenopus laevis)",
author = "Yukinao Sakai and Yoshiki Hiraoka and Masahiro Konishi and Motoyuki Ogawa and Sadakazu Aiso",
year = "1997",
month = "10",
day = "1",
doi = "10.1006/abbi.1997.0283",
language = "English",
volume = "346",
pages = "1--6",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Isolation and characterization of Xenopus laevis xSox-B1 cDNA

AU - Sakai, Yukinao

AU - Hiraoka, Yoshiki

AU - Konishi, Masahiro

AU - Ogawa, Motoyuki

AU - Aiso, Sadakazu

PY - 1997/10/1

Y1 - 1997/10/1

N2 - A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox- B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.

AB - A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox- B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.

KW - DNA binding

KW - Embryogenesis

KW - Nucleotide sequence

KW - SOX

KW - SRY (Xenopus laevis)

UR - http://www.scopus.com/inward/record.url?scp=0031260134&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031260134&partnerID=8YFLogxK

U2 - 10.1006/abbi.1997.0283

DO - 10.1006/abbi.1997.0283

M3 - Article

VL - 346

SP - 1

EP - 6

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 1

ER -