Isolation and characterization of Xenopus laevis xSox-B1 cDNA

Yukinao Sakai, Yoshiki Hiraoka, Masahiro Konishi, Motoyuki Ogawa, Sadakazu Aiso

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Abstract

A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox- B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume346
Issue number1
DOIs
Publication statusPublished - 1997 Oct 1

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Keywords

  • DNA binding
  • Embryogenesis
  • Nucleotide sequence
  • SOX
  • SRY (Xenopus laevis)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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