Isolation and expression of a human SRY-related cDNA, hSOX20

Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Koji Taniguchi, Takuma Fujii, Akihiro Umezawa, Jun Ichi Hata, Sadakazu Aiso

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Abstract

SOX is a family of genes related to the testis-determining gene, SRY. We have isolated and sequenced an hSOX20 cDNA: from a cell line of human embryonic carcinoma. This cDNA contains an open reading frame (ORF) encoding 233 amino acids. The protein encompasses an SRY-type HMG box exhibiting strong homologies to those of mouse Sox15 and Sox16. Various adult and fetal tissues were tested for hSOX20 mRNA by Northern analysis. Its expression is restricted to the fetal testis and the size of the transcript is 1.5 knt. Electrophoretic mobility shift assay indicated that recombinant hSOX20 polypeptide is capable of binding to AACAAT sequence.

Original languageEnglish
Pages (from-to)132-137
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1396
Issue number2
DOIs
Publication statusPublished - 1998 Mar 9

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Keywords

  • DNA binding
  • Embryonic carcinoma
  • Fetal testis
  • HMG box
  • SOX

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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