Abstract
Two novel ethylene-assimilating bacteria, strains ETY-M and ETY-NAG, were isolated from seawater around Japan. The characteristics of both strains were investigated, and phylogenetic analyses of their 16S rRNA gene sequences showed that they belonged to the genus Haliea. In C1-4 gaseous hydrocarbons, both strains grew only on ethylene, but degraded ethane, propylene, and propane in addition to ethylene. Methane, n-butane, and i-butane were not utilized or degraded by either strain. Soluble methane monooxygenase-type genes, which are ubiquitous in alkene-assimilating bacteria for initial oxidation of alkenes, were not detected in these strains, although genes similar to particulate methane monooxygenases (pMMO)/ammonia monooxygenases (AMO) were observed. The phylogenetic tree of the deduced amino acid sequences formed a new clade near the monooxygenases of ethane-assimilating bacteria similar to other clades of pMMOs in type I, type II, and Verrucomicrobia methanotrophs and AMOs in alpha and beta proteobacteria.
| Original language | English |
|---|---|
| Pages (from-to) | 54-60 |
| Number of pages | 7 |
| Journal | Microbes and environments |
| Volume | 27 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2011 Dec 1 |
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Keywords
- Haliea
- Particulate methane monooxygenase (pmo)
- Short-chain alkene
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Soil Science
- Plant Science
Cite this
Isolation of two novel marine ethylene-assimilating bacteria, Haliea species ETY-M and ETY-NAG, containing particulate methane monooxygenase-like genes. / Suzuki, Toshihiro; Nakamura, Takamichi; Fuse, Hiroyuki.
In: Microbes and environments, Vol. 27, No. 1, 01.12.2011, p. 54-60.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of two novel marine ethylene-assimilating bacteria, Haliea species ETY-M and ETY-NAG, containing particulate methane monooxygenase-like genes
AU - Suzuki, Toshihiro
AU - Nakamura, Takamichi
AU - Fuse, Hiroyuki
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Two novel ethylene-assimilating bacteria, strains ETY-M and ETY-NAG, were isolated from seawater around Japan. The characteristics of both strains were investigated, and phylogenetic analyses of their 16S rRNA gene sequences showed that they belonged to the genus Haliea. In C1-4 gaseous hydrocarbons, both strains grew only on ethylene, but degraded ethane, propylene, and propane in addition to ethylene. Methane, n-butane, and i-butane were not utilized or degraded by either strain. Soluble methane monooxygenase-type genes, which are ubiquitous in alkene-assimilating bacteria for initial oxidation of alkenes, were not detected in these strains, although genes similar to particulate methane monooxygenases (pMMO)/ammonia monooxygenases (AMO) were observed. The phylogenetic tree of the deduced amino acid sequences formed a new clade near the monooxygenases of ethane-assimilating bacteria similar to other clades of pMMOs in type I, type II, and Verrucomicrobia methanotrophs and AMOs in alpha and beta proteobacteria.
AB - Two novel ethylene-assimilating bacteria, strains ETY-M and ETY-NAG, were isolated from seawater around Japan. The characteristics of both strains were investigated, and phylogenetic analyses of their 16S rRNA gene sequences showed that they belonged to the genus Haliea. In C1-4 gaseous hydrocarbons, both strains grew only on ethylene, but degraded ethane, propylene, and propane in addition to ethylene. Methane, n-butane, and i-butane were not utilized or degraded by either strain. Soluble methane monooxygenase-type genes, which are ubiquitous in alkene-assimilating bacteria for initial oxidation of alkenes, were not detected in these strains, although genes similar to particulate methane monooxygenases (pMMO)/ammonia monooxygenases (AMO) were observed. The phylogenetic tree of the deduced amino acid sequences formed a new clade near the monooxygenases of ethane-assimilating bacteria similar to other clades of pMMOs in type I, type II, and Verrucomicrobia methanotrophs and AMOs in alpha and beta proteobacteria.
KW - Haliea
KW - Particulate methane monooxygenase (pmo)
KW - Short-chain alkene
UR - http://www.scopus.com/inward/record.url?scp=84863357738&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863357738&partnerID=8YFLogxK
U2 - 10.1264/jsme2.ME11256
DO - 10.1264/jsme2.ME11256
M3 - Article
C2 - 22307463
AN - SCOPUS:84863357738
VL - 27
SP - 54
EP - 60
JO - Microbes and Environments
JF - Microbes and Environments
SN - 1342-6311
IS - 1
ER -