Label-free imaging of melanoma with confocal photothermal microscopy: Differentiation between malignant and benign tissue

Takayoshi Kobayashi, Kazuaki Nakata, Ichiro Yajima, Masashi Kato, Hiromichi Tsurui

Research output: Contribution to journalArticle

Abstract

Label-free confocal photothermal (CPT) microscopy was utilized for the first time to investigate malignancy in mouse skin cells. Laser diodes (LDs) with 405 nm or 488 nm wavelengths were used as pumps, and a 638 nm LD was used as a probe for the CPT microscope. A Grey Level Cooccurrence Matrix (GLCM) for texture analysis was applied to the CPT images. Nine GLCM parameters were calculated with definite definitions for the intracellular super-resolved CPT images, and the parameters Entropy, Contrast, and Variance were found to be most suited among the nine parameters to discriminate clearly between healthy cells and malignant cells when a 405 nm pump was used. Prominence, Variance, and Shade were most suited when a pump wavelength of 488 nm was used.

Original languageEnglish
Article number67
JournalBioengineering
Volume5
Issue number3
DOIs
Publication statusPublished - 2018 Sep 1
Externally publishedYes

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Confocal microscopy
Labels
Pumps
Tissue
Imaging techniques
Semiconductor lasers
Wavelength
Skin
Microscopes
Entropy
Textures
Cells

Keywords

  • Label-free imaging
  • Melanoma
  • Photothermal microscopy
  • Texture analysis

ASJC Scopus subject areas

  • Bioengineering

Cite this

Label-free imaging of melanoma with confocal photothermal microscopy : Differentiation between malignant and benign tissue. / Kobayashi, Takayoshi; Nakata, Kazuaki; Yajima, Ichiro; Kato, Masashi; Tsurui, Hiromichi.

In: Bioengineering, Vol. 5, No. 3, 67, 01.09.2018.

Research output: Contribution to journalArticle

Kobayashi, Takayoshi ; Nakata, Kazuaki ; Yajima, Ichiro ; Kato, Masashi ; Tsurui, Hiromichi. / Label-free imaging of melanoma with confocal photothermal microscopy : Differentiation between malignant and benign tissue. In: Bioengineering. 2018 ; Vol. 5, No. 3.
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